内蒙古地区瑞香狼毒药材HPLC指纹图谱研究

HPLC fingerprint of Stellera chamaejasme of Inner Mongolia Region

目的：建立内蒙古地区瑞香狼毒药材的HPLC指纹图谱。方法：采用反相高效液相色谱法，选用AKZONOBEL KromasilC18（250mm×4．6mm，5μm）色谱柱；流动相为乙睛-0．5％磷酸溶液（梯度洗脱）；分析时间60min；检测波长为297nm，柱温：15％。结果：建立了瑞香狼毒药材的HPLC指纹图谱，标定了瑞香狼毒药材14个共有指纹峰，方法学考察结果符合指纹图谱技术要求，10批瑞香狼毒药材的相似度在0．9～1．0之间。结论：该方法稳定、可靠、重现性好，可为内蒙古地区瑞香狼毒药材质控标准的制定提供参考。

AIM： To establish a HPLC fingerprint of Stellera chamaejasme L. from Inner Mongolia Region. METHODS： The RP-HPLC method was used with Akzonobel Kromasil C18 （250 mm x4.6 mm, 5 μm） the aceto- nitrile - 0.5 % phosphoric acid （ gradient elution） was used as mobile phase, analytic time was 60 rain, and detective wavelength was at 297 nm, the column temperature of 15℃ were adopted. RESULTS： The HPLC fingerprint of Stellera chamaejasme L. set up showed that 14 peaks were co-possessing in different sources. The results of method validation met technical standard of fingerprint, the similarities of Stellera chamaejasme L. were 0.9 to 1.0. CONCLUSION： The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of Stellera chamaejasme L. from Inner Mongolia Region.