丹参中活性成分的同时定量分析及其相关性研究

Simultaneous determination and correlation study on the active constituents in Salvia miltiorrhiza Bge

目的:建立丹参药材中主要活性成分的HPLC定量分析方法,测定丹参活性成分的含量,以及探讨这些活性成分含量之间的相关性。方法:以ODS C18柱(150mm×4.6mm,10μm)为分析柱,甲醇-1%四氢呋喃的1%甲酸水溶液(3∶97)为流动相,梯度洗脱286nm为测定波长,进行丹参活性成分的HPLC分析,以spss13.0统计软件进行相关性评价。结果:按照上述方法,建立了丹参药材活性成分的定量分析方法,对其中的8个主要活性成分进行了定量分析。以spss13.0统计软件对活性成分之间含量的相关性进行了计算。结论:通过分析,发现不同产地来源的的丹参药材之间活性成分的含量差异较大;并首次发现,丹参药材中水溶性的活性成分丹酚酸B、丹参素、原儿茶醛的含量之间存在着显著的相关性;脂溶性的丹参酮Ⅰ、丹参酮Ⅱ及隐丹参酮的含量之间存在着显著的相关性;水溶性活性成分的含量和脂溶性活性成分含量之间无相关性。

AIM： To establish a simple and accurate HPLC method for quality evaluation and determination of 8 active constituents, i.e. Danshensu, Protocatechualdehyde, rosmarinic acid, Salvianolic acid B, Dihydrotanshinone, Cryptotanshinone, Tanshinone Ⅰ and Tanshinone Ⅱ A in Radix Salviae Mihiorrhizae, at the same time, in order to study the correlations of the contents of the 8 constituents. METHODS： Detected at 286 nm, analytes were separated on a ODS C18 column（ 150 mm×4.6 mm, 10 μm） eluted gradually with methanol and 1% tetrafuran in 1% formic acid water solution（3：97 ）on the increasement of methanol from 3% to 70% in the whole analy- sis. After determination, SPSS 13.0 software was used to calculate the correlation between the 8 constituents. RESULTS ： Under the above conditions, HPLC method was constructed to quantify 8 constituents, and their correlation coefficients were calculated. CONCLUSION： The result shows that the contents of the 8 constituents in Salvia miltiorrhiza Bge. vary greatly from different origins; and there are significant correlations between the contents of hydrophilic constituents such as Salvianolic acid B, Danshensu, rosmarinic acid, so do hydrophobic constituents like Cryptotanshinone,Tanshinone Ⅰ ,Tanshinone Ⅱ A, but there is no correlation between the hydrophilic constituents and hydrophobie constituents.