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辐射制备的M-PEIs基因转导载体细胞生物学研究

Cell biology of M-PEIs gene deliver prepared with radiation
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摘要 将辐射制备的M-PEIs纳米凝胶转导质粒短发夹状RNA(shRNA),研究了载体复合物的细胞毒性、shRNA转染效率及对靶基因的表达抑制。结果表明M-PEIs纳米凝胶的细胞毒性较低,可以较高基因转染效率将shRNA转移到细胞内,同时携带的质粒shRNA在靶细胞内具有较长的释放期,转染的shRNA能使肝癌细胞的靶基因失活,有效抑制了靶基因的表达,初步证明辐射制备的M-PEIs纳米凝胶是一种有效的基因治疗载体。 To study the feasibility of using polyethyleneimine nanoparticles （M-PEIs） prepared by radiation polym- erization as gene deliver, the cytotoxicity of M-PEIs was evaluated by MTS assay. The efficiency of short hairpin RNA（shRNA） transfection and its suppression of the targetted gene expression were investigated by RT-PCR and Western Blotting. It was found that the M-PEIs/shRNA complexes exhibited low cyto-toxicity in HCC. M-PEIs/shRNA also suppressed the target gene expression in liver cancer cell HepG2. The results indicate that M-PEIs have potential as a shRNA delivering agent and can be applied for anti-angiogenic tumor therapy.
作者 董黎 徐冬梅 洪军 宫培军 姚思德
机构地区 中国科学院上海应用物理研究所
出处 《核技术》 EI CAS CSCD 北大核心 2008年第6期465-468,共4页 Nuclear Techniques
基金 国家重点基础研究发展计划973项目(2006CB705700) 上海市纳米专项(0243nm027)资助
关键词 辐射制备 聚乙烯亚胺 基因载体 短发夹状RNA Radiation preparation, Polyethylenimine, Gene deliver, shRNA
分类号 Q782 [生物学—分子生物学] R329.26 [医药卫生—人体解剖和组织胚胎学]
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参考文献17

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