FasL蛋白及其相互作用蛋白对直肠癌细胞耐药性影响的研究

FasL and Its Interacted Proteins in the Drug-resistance of Colorectal Carcinoma

目的:探讨FasL蛋白及其相互作用蛋白在直肠癌细胞耐药中的作用机制。方法:利用酵母双杂交系统在相互作用蛋白研究中的特性,筛选与FasL蛋白相互作用较强的蛋白质,通过细胞学实验(MTT法)研究FasL蛋白及其相互作用蛋白对直肠癌细胞耐药性的影响。结果:经过筛选发现,FasL蛋白与anchor attachment蛋白、金属硫蛋白1K具有较强的相互作用。经细胞耐药学实验证实,FasL蛋白、anchor attachment蛋白与金属硫蛋白1K组直肠癌HR-8348细胞的耐药性明显高于其他组细胞,差异具有非常显著性统计学意义(P<0.01),FasL蛋白组以及FasL蛋白和金属硫蛋白1K组直肠癌HP-8348细胞的耐药性均高于空白对照组,差异具有显著性统计学意义(P<0.05)。结论:FasL蛋白与anchor attachment蛋白1K结合后,增强了金属硫蛋白1K在结直肠癌细胞中的耐药能力,可能促进了结直肠癌的复发与转移。

Objective：To screen the proteins which interact with FasL and investigate the mechanism of drug-resistance colorectal carcinoma. Methods： The fetal liver cDNA library was screened using the yeast double-hybrid system and a series of specific proteins that interacted with FasL protein were obtained. We used cytologic method and analyzed interacted proteins to see how they influenced the drug-resistance of colorectal carcinoma. Results ： It was found through screening that several proteins interacted strongly with FasL protein,including metallothionein 1 K, anchor attachment protein, phospholipid scramblase. MTT showed that FasL protein, anchor attachment protein and metallothionein 1K could promote the drug-resistance of HR-8348 cells of colorectal carcinoma（ P 〈 0.01 ）. Their drug-resistance was stronger than that of the control group, showing a significant difference （P 〈 0.05 ）. Conclusion： FasL, when combined with anchor attachment protein 1 K,metallothionein 1K,may prompt the drug resistance of eolorectal carcinoma and perhaps will promote the recurrence and metastasis of colorectal carcinoma.