天然与细胞培养竹节参的抗炎、镇痛作用研究

A Study of Natural and Cell Culture Panax Japonicus on Anti-inflammatory and Analgesia

目的:对比研究天然竹节参和植物细胞培养竹节参的抗炎和镇痛活性,确定细胞培养竹节参的活性成分。方法:以二甲苯致小鼠耳肿胀为急性炎症模型,以小鼠醋酸扭体实验为止痛模型,分别对天然竹节参,细胞培养竹节参及细胞培养竹节参的分离产物总皂苷、多糖等成分进行抗炎和镇痛试验。结果:细胞培养竹节参中、高剂量组对二甲苯致小鼠耳肿胀的影响与对照组比较,差异有显著性和非常显著性统计学意义(P<0.05,P<0.01);天然竹节参组及细胞培养竹节参中、高剂量组对醋酸致小鼠扭体反应的影响与对照组比较,差异有显著性及非常显著性统计学意义(P<0.05,P<0.01);竹节参分离产物总皂苷中、高剂量组对二甲苯致小鼠耳肿胀的影响与对照组比较,差异有显著性统计学意义(P<0.05);多糖组及总皂苷中、高剂量组对醋酸致小鼠扭体反应扭体次数的影响与对照组比较,差异有显著性及非常显著性统计学意义(P<0.05,P<0.01)。结论:细胞培养竹节参与野生天然竹节参的药用价值相当。

Objective：To compare the activity of natural and cell culture Panax japonicus on anti-inflammatory and analgesia and determine the active components in cell culture Panax japonicus. Methods：The natural and cell culture Panax japonicus ,general saponin and polysaccharide were studied using xylene as acute inflaminnatory model for mice ear engorgement and analgesia model for mice writhing test. Resuits：Compared with the control group, medium and high dosage cell culture Panax japonicus significantly lessened the ear engorgement caused by xylene （P 〈 0.05 and P 〈 0.01 ）. Natural and cell culture Panax japonicus inhibited the time and number of appearing writhing significantly （ P 〈 0.05 and P 〈 0.01 ）. The general saponin inhibited significantly the mice ear engorgement caused by xylene （ P 〈 0.05 ）. In the experiment of mice writhing, polysaccharide and general saponin exhibited significant differences respectively（ P 〈 0.05 and P 〈 0.01 ）. Conclusion：Cell culture Panax japonicus may act as the succedaneum of natural Panax japonicus.