摘要
采用Fmoc固相合成法,利用穿膜肽Tat对胸腺五肽(TP-5)进行修饰,合成了新的胸腺五肽类似物,总收率为32.2%.通过RP-HPLC和质谱对该化合物进行了鉴定,采用E-玫瑰花结实验进行生物活性分析,结果表明:经穿膜肽修饰的类似物在其摩尔浓度稀释20倍后仍然比标准品TP-5具有更强的生物活性.
Using the cell-penetrating peptide to modify the thymopentin at N-terminal, the novel thymopentin (TP-5) analogues have been synthesized by a Fmoe solid-phase synthesis strategy. The total yield was 32.2%. The analogues were confirmed by reversed-phase high performance liquid chromatography and mass spectrum. Their biological activity was detected by E-rose test . Results indicated that the biological activities of the analogues were stronger than standard TP-5 still when their concentrations were 20 times lower than standard TP-5.
出处
《华中师范大学学报(自然科学版)》
CAS
CSCD
2008年第2期242-246,共5页
Journal of Central China Normal University:Natural Sciences
基金
国家高技术研究发展计划(863计划)资助项目(2006AA090504)
湖南省教育厅资助科研项目(07B021)
湖南科技大学博士启动基金资助项目(E50632)
关键词
穿膜肽
胸腺五肽
固相合成
E-玫瑰花
cell-penetrating peptide
thymopentin
solid phase synthesis
E-rose