冠状动脉微栓塞后MCP-1变化的实验研究

Changes of monocyte chemotactic protein-1 after coronary microembolization

目的经导管建立急性冠状动脉微栓塞模型,检测微栓塞后单核细胞趋化因子-1 (monocyte chemotactic protein-1,MCP-1)的变化情况。方法12只小型猪,通过导管方法建立急性冠脉微栓塞模型,观察急性期(微栓塞前、微栓塞后2 h、6 h)及慢性期(微栓塞前、微栓塞后1周)血清中MCP-1浓度变化,RT-PCR观察心肌组织中MCP-1及粒细胞变化。结果MCP-1随时间变化逐渐增加,MCP-1在2 h有明显增加,与基础相比P<0.05;6 h MCP-1与基础相比P<0.01。1周时,而MCP-1与微栓塞前相比没有明显变化(P>0.05),心肌组织RT-PCR显示MCP-1mRNA表达只在6 h明显升高(P<0.05),1周时不再升高(P>0.05)。微栓塞1周后前壁心肌组织病理切片计数测得粒细胞为(103.0±49.4),后壁测得为(43.9±24.0),两者相比有显著差异性(P<0.05)。结论冠脉微栓塞后血清MCP-1呈现先升高后下降趋势,心肌组织MCP-1mRNA只在微栓塞后6 h升高。

Objective To investigate changes of monocyte chemotactic protein-1 （ MCP-1 ） after coronary mieroembolization （CME）. Method The left anterior descending coronary artery of 12 pigs was embolized by injection of 42 m microspheres with a total of 120,000. Study was divided as acute study （ base, 2 hours and 6 hours） and chronic study （ base and 1 week）. Serum MCP-1 was measured by ELISA. MCP-1mRNA from myoeardium was analyzed with RT-PCR. Leukoeytes from myocardium were counted. Results MCP-1 decreased significantly after CME （34.64 ± 2.71 pg/ml at baseline, 41.24 ± 2.88 pg/ml at 2 hours and 42.97 ± 3.54 pg/ml at 6 hours, 2 hours and 6 hours compared with base P 〈 0.05 and P 〈 0.01 respectively）. At one week later, MCP-1 returned to baseline （35.33 ± 2.37 pg/ml at base and 34.92 ±3.49 pg/ml at l week）. RT-PCR analysis showed that MCP-1mRNA only elevated at six hours after CME （P 〈 0.05 ）. Number of leukocyte infiltration was 103.0 ± 49.4 at anterior myocardial and 43.9 ± 24.0 at posterior（P 〈 0.05）. Conclusion Changes of serum MCP-1 were first decreased then returned to baseline. MCP-1 mRNA only elevates at six hours after CME.