1536份临床标本细菌培养与药敏结果分析

Bacteria culturing and antimicrobial susceptibility results analysis of 1536 clinical specimens

目的了解本医院2006年度部分临床标本的细菌感染分布及其药物敏感情况。方法按照临床致病菌的培养与鉴定及药物敏感试验方法，对我院2006年门诊及住院病人的血液、分泌物、胆汁、中段尿共计1536份常见标本按照常规方法进行培养、分离、鉴定与药物敏感试验。结果共分离出致病菌503株，其中血液标本1025份，分泌物143份，中段尿193份，胆汁标本175份，检出率分别为10．6％、87．4％、76．0％、70．5％。药敏试验显示阿米卡星对革兰阴性菌最为敏感，其敏感率为89．9％，其次为头孢他啶，敏感率为85．3％，万古霉素对革兰阳性菌的敏感率高达98．4％，其次为环丙沙星，敏感率达86．6％。结论了解常见致病菌的分布与检出及药敏情况，结合临床，达到合理、安全指导临床选用抗生素，确保治疗的目的。

Objective To investigate the distribution and antimicrobial susceptibility of clinical bacterial isolates in the hospital in 2006. Methods 1536 specimens, including blood, secreta, bile, middlestream voided urine, were collected for bacteria culture. Bacterial isolates were identified by conventional method. The antimicrobial susceptibility test was performed with the dilution method. Results 503 strains of pathogenic bacteria were isolated from 1025 blood samples, 143 secreta samples, 175 bile samples, 193 middlestream voided urine samples. The positive rate were 10. 6% , 87.4% , 76. 0% , and 70. 5% respectively. The antimicrobial susceptibility results showed amikacin was most susceptible to Gram- negative isolates （89.9%） , followed by cefiazidime （85.3%） , while vancomycin was most susceptible to Gram- positive isolates （98.4%） , followed by ciprofloxacin （ 86. 6% ）. Conclusion For the rational use of antimicrobial agents, it is important to evaluate the distribution and drug resistance of pathogenic bacteria.