芒果苷原料药中杂质高芒果苷的含量测定

Determination of Impurity of Homomangiferin in Raw Medicine of Mangiferin by HPLC

目的分析测定芒果苷原料药中高芒果苷的含量。方法采用C18柱（大连依利特分析仪器有限公司，Hypersil ODS 5μm，4．6mm×250mm），流动相为甲醇-0．1％磷酸（32：68），流速1ml／min，检测波长258nm。结果高芒果苷在0．0424-0．848μg范围内进样量与峰面积呈良好的线性关系（r=0．9999），平均回收率为97．8％，RSD=2．2％（n=6）。结论该方法简便，灵敏，重复性好，可作为芒果苷原料药中高芒果苷的含量测定方法。

Objective To determine Homomangiferin in raw medicine of Mangiferin by HPLC. Methods Homomangiferin was separated on Hypersil ODS 5μm column （4.6 mmID × 250mm） with a mobile phase of methanol -0. 1% phosphoric acid solution （32：68 ） and detected at a wavelength of 258 nm. Results The calibration curve was linear in the range of 0.042 4 - 0. 848 μg （r = 0.999 9）. The average recovery rate was 97.8% and RSD = 2.2% （n = 6）. Conclusion The method is simple, sensitive and precise. It can be used for the determination of Homomangiferin in raw medicine of Mangifierin.