不同产地的知母中总皂苷和菝葜皂苷元的含量测定

Determination of Anemarrhena Saponin and Sarsa Sapogenin in Anemarrhena from Various Habitats

目的考察不同产地知母中总皂苷、菝葜皂苷元的含量。方法采用香草醛-高氯酸法测定不同产地知母中总皂苷的含量；采用高效液相色谱-蒸发光散射法测定不同产地知母中菝葜皂苷元的含量，色谱柱Waters Nova—Pak C18；流动相为甲醇：水=90：10；流速为1．0ml·min^-1；柱温室温，漂移管温度45℃；载气流速30psi。结果河北、安徽、广东知母中总皂苷的含量为6．04％～7．91％，回收率为99．28％，RSD为2．83％（n=6）；菝葜皂苷元的线性范围为1．65～6．6μhg（r=0.9997），河北、安徽、广东知母中的菝葜皂苷元含量为0．72％～0．97％，回收率为106．34％，RSD为2.43％（n=6）。结论各产地知母中菝葜皂苷元含量间变异较大，以河北知母中总皂苷及菝葜皂苷元的含量最高。

Objective To determine the content of Anemarrhena saponin and sarsa sapogenin in Anemarrhena. Methods The content of Anemarrhena saponin was determined by colorimetry ; the content of sarsa sapogenin was determined by HPLC - ELSD. The column was a packed with 5 μm stationary phase Waters Nova -Pak C18 column and a mobile phase of methanol -water （90 ： 10） , with a flow rate of 1.0 ml · min^-1 , at a temperature of drift tube of 45℃, and the nebulizer nitrogen flow rate was 30 psi, with a universal detector Evaporation Light Scattering Detector （ELSD）. Results Anemarrhena saponin in Anemarrhenae was 6.04% - 7.91% , and the recovery rate was 99.28 % , RSD 〈 3% （ n = 6 ）. The sarsa sapogenin calibration curve was linear in the range of 1.65 - 6.6 μg （ r = 0. 999 7 ） and the recovery rate was 106.34% , RSD was 2.43% （ n=6 ）. Conclusion There are great changes among the different originations. The content of Anemarrhen asaponin and sarsa sapogenin in hebei Anemarrhena is the most.