摘要
目的研究用高效液相色谱测定女贞子中齐墩果酸含量的方法,并与薄层扫描色谱法比较。方法色谱柱为kromasil C18(5μm,250mm×4.6mm);柱温30℃;流动相甲醇-水(85:15);流速0.7ml·min^-1;检测波长为204nm。结果齐墩果酸在0.6456~4.304μg范围内线性关系良好,r=0.9999(n=5),平均回收率为101.8%,RSD=0.75%。结论高效液相色谱法比薄层扫描色谱法更灵敏、简便、准确,精密度好。
Objective To establish an HPLC method for the determination of the content of oleanolic acid , and compare the resuits by HPLC and TLCS. Methods With a kromasil C18 (5 μm,250 mm×4.6 mm) at 30℃, methyl alcohol -water(85: 15) was used as the mobile phase ,the flow rate was 0.7ml/min ,the wavelength was 204 nm. Results The oleanolic acid had a good linear relationship with HPLC in the range of 0.645 6 -4. 304μg , r = 0. 999 9. The average recovery was 101.8% and RSD was 0.75%. Conclusion It is proved that the HPLC is more reliable , simple, accurate and reproducible than TLCS is.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第6期1411-1412,共2页
Lishizhen Medicine and Materia Medica Research