高效液相色谱法与薄层扫描法测定女贞子中齐墩果酸含量的比较

A Comparison of HPLC with TLCS in Determination of Oleanolic Acid

目的研究用高效液相色谱测定女贞子中齐墩果酸含量的方法，并与薄层扫描色谱法比较。方法色谱柱为kromasil C18（5μm，250mm×4.6mm）；柱温30℃；流动相甲醇-水（85：15）；流速0．7ml·min^-1；检测波长为204nm。结果齐墩果酸在0．6456～4.304μg范围内线性关系良好，r=0．9999（n=5），平均回收率为101．8％，RSD=0.75％。结论高效液相色谱法比薄层扫描色谱法更灵敏、简便、准确，精密度好。

Objective To establish an HPLC method for the determination of the content of oleanolic acid , and compare the resuits by HPLC and TLCS. Methods With a kromasil C18 （5 μm,250 mm×4.6 mm） at 30℃, methyl alcohol -water（85： 15） was used as the mobile phase ,the flow rate was 0.7ml/min ,the wavelength was 204 nm. Results The oleanolic acid had a good linear relationship with HPLC in the range of 0.645 6 -4. 304μg , r = 0. 999 9. The average recovery was 101.8% and RSD was 0.75%. Conclusion It is proved that the HPLC is more reliable , simple, accurate and reproducible than TLCS is.