薄层扫描法测定金蝉口服液中黄芩苷的含量

Determination of Baicalin in Jinchan Oral Liquid by TLC Scanning

目的:测定金蝉口服液中黄芩苷的含量,建立金蝉口服液的质量控制方法。方法:用聚酰胺薄膜分离金蝉口服液中黄芩苷,薄层扫描法测定其含量;以φ(C_2H_4O_2,HAC)=36%作展开剂;采用双波长反射法锯齿扫描,检测波长(λ_s=278 nm,λ_R=370 nm)。结果:黄芩苷点样量在0.217～1.083μg/mL范围内呈良好的线性关系,回归方程:A=2 131.551+7.545 C,r=0.999 3,平均加样回收率:101.60%,RSD=2.50%(n=5)。结论:该方法准确、简便,适合该制剂中黄芩苷的含量测定。

Objective： To establish a method for quality control of Jinchan oral liquid, the content of Baicalin in Jinchan oral liquid was determined by thin layer chromatographic scanning（TLCS）. Methods： Baicalin of Jinchan oral liquid was separated by φ（C2H4O2, HAC）= 36% acids in polyamide film; the method of the dual wavelength TLCS was adopted to determine the content of baicalin in Jinchan oral liquid and the detection wave-length was λs =278 nm, λR = 370 nm. Results： The content of baicalin between 0.217 M.083μg/mL showed a good linearity. The equation of linear regression was A = 2 131.551＋ 7.545 C, r = 0.999 3. The average recovery was 101.60% and RSD was 2.50%（n = 3）. Conclusion： This method is accurate and simple. It can be used to determine baicalin in dinchan oral liquid.