黄河中游地区粗山羊草高分子量谷蛋白亚基组成分析

The Analyse of Allelic Composition of the HMW Glutenin Subunits in Aegilops tauschii in Middle Reaches of the Yellow River

为了解黄河中游地区粗山羊草的高分子量谷蛋白亚基(HMW-GS)遗传多样性,应用SDS-PAGE技术分析了56份粗山羊草Glu-Dt1位点的HMW-GS组成,检测到3种x-型亚基(5t,6.2t,null)和3种y-型亚基(10.5t,10.4t,10.3t)。其中6.2t类型是新的x-型亚基。供试粗山羊草共发现3种HMW-GS组合类型5t+10.5t,6.2t+10.4t,null+10.3t,它们的比例分别是91.07%,7.14%,1.79%。表明该地区粗山羊草的高分子量谷蛋白亚基有一定变异。

A Collection of 56 accessions of Aegilops tauschii was investigated for the allelic composition of the high molecular weight subunits at the Glu-Dtl locus by SDS-PAGE analysis. Three x-type glutenin subunits（5t ,6.2t, null）and Three y-type subunits （ 10.5t, 10.4t, 10.3t ） were identified, respectively, according to their ascending mobility. Among them, subunit 6.2t was a novel x-type glutenin subunit. Three HMW-GS combinations （5t ＋ 10.5t, 6.2t ＋ 10.4t, null ＋ 10.3t, ） were found in these collection, of which their percent were 91.07 %, 7.14 % and 1.79 %. The results indicated that some genetic diversity were presented in the 56 accessions of Aegilops tauschii.