早期心肌缺血猝死心肌Ⅰ型胶原的表达

Expression of Myocardial CollagenⅠin Early Acute Myocardial Ischemic of Human

目的探讨Ⅰ型胶原蛋白在心肌早期缺血死后诊断中的法医学价值。方法将收集的案例心肌蜡块分为3组:正常对照组、早期心肌缺血组、心肌梗死组,用免疫组织化学技术(S-P法),观察猝死心肌内Ⅰ型胶原的表达情况,对其结果进行半定量分析。结果早期心肌缺血组和心肌梗死组心肌细胞质以及细胞核染色阳性,且早期心肌缺血组与心肌梗死组表达强度相近,说明早期心肌缺血组在胶原网结构方面已经发生与心肌梗死相同的改变。结论Ⅰ型胶原可以作为早期心肌缺血的一个诊断指标。

Objective To estimate the significance of myocardial collagen Ⅰ in the early acute myocardial ischemia of human. Methods The myocardial paraffin block had normal group, early myocardial ischemia group and myocardial infarction group. The myocardial collagen Ⅰ was observed with immunohistochemical staining and analyzed by halfquantity. Results The similar expression of collagen I was observed in the cytoplasm and nucleolus in two groups. Conclusion The collagen I appeared in the myocardium of early acute myocardial ischemia of human. It may be an important index for postmortem diagnosis of the early acute myocardial ischemia.