不同免疫抑制剂对人膀胱肿瘤细胞抑制作用的比较研究

Comparison of inhibitive effects of different immunosuppressive agents on human bladder cancer cell lines in vitro

目的观察常用免疫抑制剂(环孢素A、霉酚酸酯、他克莫司、雷帕霉素)对人膀胱癌细胞系T24和5637的体外抑制作用,并比较其作用强弱。方法MTT法测定各实验组细胞吸光度值,以观察不同药物对两种肿瘤细胞生长的抑制作用;侵袭小室法测定经药物处理后穿透生物膜的细胞数,以观察药物对细胞侵袭力的抑制效应。结果对T24细胞24h抑制率(%)分别为:环孢素A33.66±2.08,霉酚酸酯25.13±1.15,他克莫司41.16±2.23,雷帕霉素41.79±3.55。对5637细胞24h抑制率(%)分别为:环孢素A39.00±1.30,霉酚酸酯25.25±4.58,他克莫司32.38±2.34,雷帕霉素39.89±1.07。T24细胞经各药物处理后穿透生物膜的细胞数分别为:环孢素A13.25±1.88,霉酚酸酯18.31±2.41、他克莫司11.44±2.10、雷帕霉素11.50±1.93。5637细胞经各药物处理后穿透生物膜的细胞数分别为:环孢素A8.31±1.85,霉酚酸酯11.19±1.83、他克莫司10.19±1.22、雷帕霉素7.38±2.09。结论现有的常用免疫抑制剂对人膀胱肿瘤细胞均有明显抑制作用,以雷帕霉素作用较强。

Objective To compare the inhibitive effects of immunosuppressive agents（cyclosporin A,mycophenolate mofetil,tacrolimus, rapamycin）on human bladder cancer cell lines T24 and 5637 in vitro. Methods MTT and Transwell chamber method were used to observe the inhibitive effects of the drugs on the growth and invasiveness of human bladder cancer cells in vitro,respectively. Results The inhibitive rates of T24 cell growth were （33.66±2.08）%,（25.13±1.15）%,（41.16±2.23）%,（41.79±3.55）% after treated with cyclosporin A,mycophenolate mofetil,tacrolimus,rapamycin for 24 h,respectively.Theinhibitive rates of cell growth of 5637 were （39.00±1.30）%,（25.25±4.58）%,（32.38±2.34）%,（39.89±1.07）% after treated with cyclosporin A,mycophenolate mofetil,tacrolimus, rapamycin for 24 h,respectively.The numbers of membrane-permeating T24 cell were 13.25±1.88,18.31±2.41,11.44±2.10 and 11.50±1.93 after treated with cyclosporin A,mycophenolate mofetil,tacrolimus, rapamycin,respectively.The numbers of membrane-permeating 5637 cell were 8.31±1.85,11.19±1.83,10.19±1.22,7.38±2.09 after treated with cyclosporin A,mycophenolate mofetil,tacrolimus,rapamycin,respectively. Conclusion The common immunosuppressive agents can inhibit the growth and invasiveness of bladder cancer cell lines in vitro,and rapamycin is the more potential drug.