地塞米松药物棉片对早期兔角膜碱烧伤的疗效观察

Effect of self-made dexamethasone cotton placed in conjunctival sac in early corneal alkali burns in rabbits

目的观察结膜囊内放置地塞米松药物棉片与常规点眼比较,治疗兔角膜碱烧伤的疗效差异。方法选取16只成年家兔,随机分为点眼组和棉片组,每组8只,制作右眼角膜碱烧伤模型。点眼组为地塞米松点眼联合结膜下注射维生素C;棉片组为结膜囊内放置浸有地塞米松药液的棉片联合结膜下注射维生素C。碱烧伤后0,12,24,36,48,60h角膜荧光素钠染色,计算角膜上皮修复率;不同时点制作病理切片,观察光镜和电镜下角膜的组织病理学改变。结果角膜碱烧伤后12h,24h,36h,48h,60h棉片组与点眼组角膜上皮修复率比较,差异均有统计学意义(P<0.05),棉片组角膜上皮修复率均较点眼组高。病理组织学结果显示:棉片组中性粒细胞细胞浸润较点眼组轻,角膜上皮修复较好且基质胶原纤维排列较整齐。结论结膜囊内放置地塞米松药物棉片组治疗角膜碱烧伤,其效果优于点眼组。

Objective To compare the effect of self-made dexamethasone cotton and normal eye-drops on corneal alkali burns in rabbits. Methods Sixteen adult rabbits were chosen to establish alkali burn model,and were randomly divided into two groups：eye-drop group and medcine cotton group.Corneal fluorescein staining was used to determine repair rate of corneal epithelium at hour 0,12,24,36,48,60 after alkali burn.Pathological changes under light and electron microscope were observed at different time points. Results The repair rate was higher in medcine cotton group than in eye-drop group at hour 12,24,36,48,60 after alkali burn（P〈0.05）.Compared with that of eye-drop group,corneal epithelium repair in medcine cotton group was better,and the collagen fibers in stroma ranged regularly. Conclusion Effect of self-made dexamethasone cotton placed in conjunctival sac on early corneal alkali burns in rabbits is superior to that of eye-drops.