广西甜茶的抗过敏作用研究

Anti-allergic Effects of Rubus suavissimus Extract

目的:研究广西甜茶(Rubus suavissimusS.Lee)提取物的抗过敏作用并观察其对大鼠腹腔肥大细胞释放组胺的影响,探讨可能的作用机制。方法:采用2,4-二硝基氟苯(DNFB)及绵羊红细胞(SRBC)诱发的小鼠迟发型过敏反应,小鼠异种被动皮肤过敏反应(PCA),观察连续口服给予广西甜茶提取物的抗过敏作用。利用高效液相-库仑阵列电化学检测系统(HPLC-ECD)测定Compound 48/80刺激大鼠腹腔肥大细胞释放组胺,评价广西甜茶提取物对肥大细胞释放组胺的影响。结果:与模型组相比,连续口服广西甜茶提取物125、250及500 mg/kg均能显著抑制由DNFB诱发小鼠耳肿胀及血管通透性的增高。此外,广西甜茶提取物可以减轻小鼠异种被动皮肤过敏反应及对SRBC诱发小鼠迟发型过敏反应足跖肿胀也显示有一定程度的抑制作用。体外实验证明广西甜茶提取物对Compound 48/80刺激大鼠腹腔肥大细胞释放组胺呈剂量依赖性抑制作用。结论:广西甜茶具有显著的抗过敏作用,其作用机制可能与抑制肥大细胞释放组胺有关。

Objective：To investigate the effects of Rubus suavissimus S.Lee extract（RSE）on mice,and its influence on release of histamine from rat peritoneal mast cells induced by compound 48/80 in vitro.Methods：To establish murine delayed-type-hypersensitive model induced by 2,4-dinitrofluotobenzene（DNFB） and sheep red blood cell（SRBC）;murine type I allergic reaction induced by PCA,and then to observe the anti-allergic effect of RSE.HPLC-ECD methods were performed to detect the contents of histamine released from rat peritoneal mast cells induced by compound 48/80 in vitro.Results：Compared with the model group,RSE could ameliorate the ear swelling and capillary permeability in mice induced by DNFB and PCA.RSE also reduced the thickness of the mice hind paw induced by SRBC in significant dose-dependent manner.Meanwhile,RSE obviously inhibited the release of histamine in rat peritoneal mast in vitro,a dose-dependent manner.Conclusion：RSE exerts potential anti-allergy effects and the mechanisms may be partly related to its inhibitory effect on the release of histamine from mast cells.