山茱萸炮制前后多糖对小鼠免疫功能的影响

Effect of Polysaccharides in Crude and Processed Cornus officinalis on the Immunologic Function of Mice with Immunosuppression Induced

目的:研究山茱萸生品和制品多糖对免疫低下小鼠免疫功能的影响,以阐明其炮制增效的机理。方法:腹腔注射环磷酰胺制备免疫低下的小鼠模型,以碳粒廓清法测定非特异性免疫功能,血清溶血素测定法测定体液免疫功能,脾淋巴细胞增殖试验测定细胞免疫功能。结果:山茱萸生品和制品多糖均可提高免疫低下小鼠的碳粒廓清指数K和吞噬指数α,增加血清HC50值,明显改善免疫低下小鼠的脾淋巴细胞增殖反应,且制品多糖的作用显著优于生品多糖。结论:山茱萸生品多糖和制品多糖对免疫低下小鼠的非特异性免疫、体液免疫以及细胞免疫功能均有明显促进作用,且山茱萸经酒蒸制后,其多糖的药效显著增强。

Objective：To investigate the effect of polysaccharides in crude and processed Cornus officinalis on the immunologic function of mice with immunosuppression induced.Methods：The immunosuppressed mice were induced by Cyclophosphamide.Non-specific immune function was determined by cleaning carbon particle method.Humoral immunity was determined by serum haemolysin formation method.Cellular immunity was determined by proliferation and transformation of spleen lymphocyte method.Results：The polysaccharides in crude and processed Cornus officinalis both markedly increased the carbon particle clearance index K,phagocytic index α,serum HC50 and proliferation and transformation of spleen lymphocyte,and the polysaccharides in processed Cornus officinalis was better than the crude one.Conciusion：The polysaccharides in crude and processed Cornus officinalis have an enhanced effect on nonspecific immunity,specific humoral immunity and specific cellular immunity in immunodeppressed mice,and after being processed with wine,the action of polysaccharides increased markedly.