絮凝剂对紫菜酶解单细胞的絮凝作用及对其生长的影响

THE EFFECT OF FLOCCULATING AGENTS ON ENZYMATICALLY ISOLATED Porphyra CELLS AND THEIR INFLUENCE ON CELLS DEVELOPMENT

就解决紫菜养殖和制饵的生产实践中出现的单细胞收集困难问题进行了初步研究。首次尝试利用细胞絮凝剂收集大型海藻的酶解单细胞 ,检查了几种常规絮凝剂及新型生物絮凝剂对其絮凝作用 ,观察了对其生长发育的影响。实验中发现常规絮凝剂明矾具有明显的絮凝作用 ,浓度过高时有致畸作用 ;饱和石灰水和甲壳胺絮凝效果较好 ,但甲壳胺具有阻碍细胞发育的特点 ;Ca2 +Mg2 +基本无絮凝作用 ;Fe3+在高浓度时有致畸作用。

This is the preliminary study which resolves the problom of cell collection in Porphyra breeding and diet preparation. Several normal flocculating agents and one new biological agent were firstly used to collect the isolated cells of seaweed. The flocculation effect and the influence on cells development were investigated. The normal agent KAI(SO 4) 2 was found to be highly effective but to cause cell deformation under high concentration. Ca(OH) 2 and Chitosan were effective. But the biological agent Chitosan could hinder the development of cells. CaCl 2 and MgCl 2 had not apparent flocculent effect. Fe 3+ could raise the development rate of cells under high concentration.