DR5、DcR2在腰椎间盘中的表达及其意义

Expression of DR5 and DcR2 in Human Lumbar Intervertebral Disc

目的观察DR5、DcR2在腰椎间盘组织中的表达分布及其意义。方法采用免疫组化技术检测腰椎间盘突出症60例、正常成人腰椎间盘22个(8例);实时荧光定量RT-PCR技术检测腰椎间盘突出症30例、正常成人椎间盘9个(3例)中DR5和DcR2的表达。结果免疫组化检测显示,腰椎间盘突出症患者DR5细胞阳性率为41.60%;正常腰椎间盘组细胞阳性率为26.09%,两者间的差异有非常高度显著性意义(P=0.001);DR5mRNA定量分析进一步支持免疫组化结果(P=0.025)。DcR2蛋白和mRNA表达两组间的差异无显著性意义。结论腰椎间盘组织中存在DR5/TRAIL诱导的凋亡途径。

Objective To investigate the expression of DR5 and DcR2 protein and mRNA in lumbar intervertebral disc （LID） of patients with prolapse of LID and normal controls. Methods The expression and distribution of DR5 and DcR2 protein were immunostained in prolapsed LIDs of 60 patients and 22 normal LIDs of 8 normal controls. In parallel, mRNA of DR5 and DcR2 was quantified by real time fluorescent reverse transcriptase polymerase chain reaction （RT PCR） in prolapsed LIDs of 30 patients and 9 normal LIDs of 3 normal controls. Results The positive rates of DR5 protein in prolapsed IVDs and normal IVDs were 41.60% and 26.09% respectively. There were a significant difference between the two groups （P=0. 001）. Further similar evidences were obtained by quantification of DR5 mRNA （P=0. 025）. There was no difference in the expression of DcR2 protein and mRNA between the two groups. Conclusion The apoptosis pathway induced by DR5/tumor necrotic factor-related apoptosis-inducing ligand （TRAIL） maybe exists in LID tissues.