摘要
目的:通过研究,建立HPLC法测定降脂减肥片中葛根素含量的方法。方法:高效液相色谱法,以Lichrospher C_(18)(200×4.6mm,5μm)为色谱柱,甲醇-水(25:75)为流动相、流速1.0ml/min,检测波长250nm。结果:葛根素线性范围为0.09~0.46μg,r=0.9999;平均加样回收率为96.70%、RSD=1.21%。结论:该方法便捷、准确,可有效提高降脂减肥片质量标准的可控性。
Objective:Through the research ,to establish a method of HPLC for determination the oontent of Puerarin in Jiangzhijianfei tables. Methods: HPLC, The Liebrespher C 18 column ( 200mm × 4. 6mm ,5 μm) was used; the methanol - water(25: 75 ) as mobile phase, the flow rate was 1.0ml/min, the detection wavelength was 250nm. Results: The linear range of Puerarin was 0. 09 - 0. 46 μg ( r = 0. 999 9 ), the average recoverie was 96.7 % (RSD = 1.2% ). Conclusion: The method is simple and rapid and the result is accurate,it can more effective control the quality specification of Jiangzhijianfei tablets.
出处
《中国医药导刊》
2008年第3期452-453,共2页
Chinese Journal of Medicinal Guide
关键词
降脂减肥片
HPLG
葛根素
含量测定
Jiangzhijianfei Tablet; HPLC
Puerarin
Content determination