摘要
采用ELISA方法测定了亲和组合与非亲和组合玉米叶片内钙调素(Calmodulin,CaM)浓度的变化,旨在明确不同组合中CaM的变化趋势以及CaM是否参与玉米体内由大斑病菌HT-毒素所引发的信号转导途径。通过观察CaM特异性抑制剂三氟拉嗪(Trifluoperazine,TFP)对玉米叶片上HT-毒素诱导的R型病斑的影响,明确TFP是否可抑制玉米的抗病反应。结果发现,玉米受到HT-毒素胁迫后,叶片内CaM浓度迅速上升,总的趋势是亲和组合中的CaM曲线多呈平台状或单峰状,而非亲和组合中的CaM曲线多呈双峰状,在不同组合中CaM的变化差异显著;TFP可使叶片病斑由抗病的R型病斑转变为感病的S型病斑,说明TFP可抑制玉米的抗病反应。推测,由玉米大斑病菌HT-毒素激发的防卫反应信号转导途径与CaM密切相关。
The contents of CaM in corn plants with different Ht genes increased quickly when corn plants were treated by HT- toxins from different race isolates of Exserohilum turcicum. In general,there is one ascending peak in compatible combinations but two ascending peaks in incompatible combinations. Exposing to Trifluoperazine, one of the depressor of CaM, then treated by HT-toxins in vitro, Resistant-type lesion on incompatible corn leaves would turn into Susceptible- type lesion, like on compatible corn leaves. It was suggested that TFP could hinder the resistant reaction, in other words, CaM could play an important role in signal transduction of resistant reaction in interaction between corn and Exserohilum turcicum.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2007年第5期4-7,共4页
Journal of Hebei Agricultural University
基金
国家自然科学基金资助项目(30471126)
河北省自然科学基金资助课题(303208)
关键词
玉米大斑病
毒素
钙调素
信号转导
Northern leaf blight of corn
oxin
CaM
signal transduction
