预培养在刺槐试管苗生根中的作用

The role of preculture in rooting of Robinia poseudoacaci L.in vitro

为解决不同品种刺槐工厂化育苗中试管苗生根困难的问题,研究了培养方式以及激素浓度、不同激素配比对5个刺槐无性系试管苗生根的影响。结果表明:不同的无性系之间试管苗生根率有显著差异,且与在含有高浓度生长素的培养基上预培养的天数以及培养基中激素的种类与浓度有关,其中在培养基1/2MS+IBA 20～35 mg/L上培养4～7 d,然后转入1/2MS培养基培养Rcr、4N、2488各无性系,生根率均达97%以上,移栽成活率大于95%;2428、2466生根率为90%～92%,移栽成活率在85%以上;1/2MS+IAA 2.0 mg/L培养基可直接诱导刺槐无性系Rcr、4N试管苗生根,生根率100%、移栽成活率90%以上;2428、2466不适合直接诱导生根。

Influences of the culture pattern and concentration of plant hormone and their combinations on the five Robinia poseudoacaci L. clones rooting in vitro were systematically studied. The results showed that the rate of rooting was significantly different between different clones. The days of precultured in medium which contains elevated concentration of auxin and categories of plant growth regulators in medium are important factors that influence the percentage of contains rooting. All clones were cultured 4- 7 d in 1/2MS medium supplemented with 20- 35 mg/L IBA, then transfered to 1/2MS0 medium. For the clones Rcr,4N and 2488, rooting rate was up to 97% and transplant survival rate was up to 95 %. Whereas for the clones 2428 and 2466, rooting rate could reach 90 % - 92 %, and transplant survival rate was up to 85 %. The medium supplemented with 2.0 mg/L IAA could induce 100 % shoot of clones Rcr and 4N rooting , and transplant survival rate was as high as to 90 %. Both of clones 2428 and 2466 in vitro were unsuitable for inducing rooting in the medium supplemented with low concentration of auxin between 0.5 to 2 mg/L.