玉米耐盐愈伤组织变异体筛选体系的建立

Establishment of selection system for salt-tolerant callus variants screening in maize

为了寻找离体筛选耐盐作物类型的新途径,特选配了4个适于幼胚培养的玉米杂交组合,以其幼胚为外植体,诱导出胚性愈伤组织。继代5次后,用200 mmol/L的NaCl和5.5 mmol/L的Hyp连续筛选3次,获得耐盐愈伤组织变异体。试验确定了适于玉米幼胚培养的诱导培养基和分化培养基,比较了2种筛选剂的选择效果:用Hyp筛选得到的愈伤组织,其分化频率高于用NaCl筛选的愈伤组织;Hyp的选择频率是NaCl的两倍,表明用Hyp间接筛选玉米耐盐变异体,是一条较为合适的途径。

The objective of this experiment was to explore a suitable method to screening salt-tolerant variants in crops. The embryogenie calli were induced from immature embryos of 4 hybrid between inbred lines of maize which were eligible for immature embryo culture. After 5 generations of subculture, salt-tolerant variants were obtained through three generations continuous selections followed with 200 mmol/L of NaCI and 5.5 mmol/L of Hyp. The suitable induction medium and differentiation medium were established for immature embryos culture of maize in this study. The selection effects were compared between these two kinds of selection reagents： The differentiation frequencies of ealli selected by Hyp were higher than by NaCl, the selection frequency of Hyp was two times as much as that of NaCI. So the suitable method for selecting maize salt-tolerant variants was selected by Hyp indirectly.