利用SSR标记对12个啤酒大麦品种的聚类分析和主坐标分析

Cluster and principal coordinate analysis among 12 beer barley by SSRs

为啤酒大麦育种实践提供可靠的信息,采用SSR标记技术对12个已知来源的啤酒大麦品种的遗传背景进行了遗传多样性分析。从筛选出的25对有多态性的SSR引物中共扩增出160个等位位点,其中136个位点有多态性,占85.0%,每对引物可扩增出1-13个位点,平均5.44个等位位点。聚类分析表明,在SM相似系数0.63水平上12个啤酒大麦品种可聚成3类2组。主坐标分析将其分为2类4组。2种分类方法所获得结果基本一致,但主坐标分析更能较为真实的反映12个啤酒大麦品种间的亲缘关系。

The genetic relationships among 12 beer barley varieties with known origin were analyzed by SSRs to provide credible genetic information in breeding beer barly. 160 distinctive loci in total were amplified from 25 pairs of SSR primers, and 136 were polymorphic, which accounted for 85.0%. 1 to 13 polymorphic loci could be amplified from each primer, with an average of 5.44. The 12 beer barley varieties could be classified into 3 groups and 2 subgroups at the level of SM = 0.63 by cluster analysis, while 2 groups and 4 subgroups could be sorted by principal coordinate analysis. The two methods above exhibited similar phylogenesis among the tested cultivars. However, more authentic genetic relationship among the 12 beer barley varieties could be provided by the principal coordinate analysis.