期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

硝酸银诱导的大豆子叶中大豆Kunitz胰蛋白酶抑制剂的研究

Study on the soybean Kunitz trypsin inhibitor(SKTI) induced by silver nitrate
下载PDF
导出
摘要 采用二维电泳技术对硝酸银诱导的大豆子叶微粒体膜蛋白进行分离,利用四极杆-飞行时间串联质谱,对仅存在于诱导样品而不存在于对照样品中的蛋白质点进行氨基酸序列测定和分析,以期研究硝酸银处理对大豆子叶微粒体蛋白质的影响。结果显示:硝酸银可以诱导大豆Kunitz胰蛋白酶抑制剂(SKTI)的产生,在诱导处理的子叶中大量存在其成熟形式,也存在其5 kDa小亚基,小亚基含有“GIGTIISSPER”和“FIAEGNPLR”氨基酸序列。该研究结果为SKTI的深入研究提供了实践依据,并为其分离纯化提供了简便途径,从而有利于对其更多性质的进一步研究。 Soybean cotyledons were induced by silver nitrate. Microsomal membrane bounded proteins were separated by two-dimensional electrophoresis （2DE）. Effect on microsomal proteins of silver nitrate was studied in the work. Protein spots that existed in the induced sample but not in the control were detected and analyzed by quadrupole-time of flight/mass spectra （Q- TOF/MS/MS）. The findings bindicate that soybean Kunitz trypsin inhibitor （SKTI） can be induced by silver nitrate. There was plenty of mature SKTI and less 5 kDa subunit in the induced cotyledons. The 5 kDa subunit includes two amino acid sequence ＂GIGTIISSPER＂and＂FIAEGNPLR＂. The results supplied practical foundation and simple separation method for the further study of SKTI.
作者 乞永艳 刘富海 Arnaud Bovy Jan Corde wener Froukje vander wal
机构地区 农业部规划设计研究院农业发展与投资研究所 北京中健高科科技中心 Plant Research International
出处 《河北农业大学学报》 CAS CSCD 北大核心 2007年第4期19-23,共5页 Journal of Hebei Agricultural University
基金 荷兰瓦赫宁根大学"交叉研究与教育基金"
关键词 大豆 硝酸银 诱导 Kunitz胰蛋白酶抑制剂 二维电泳 四极杆-飞行时间串联质谱 soybean silver nitrate induce Kunitz trypsin inhibitor two-dimensional electrophoresis quadrupole-time of flight/mass spectra
分类号 S565.1 [农业科学—作物学]
  • 相关文献

参考文献28

  • 1大豆中胰蛋白酶抑制剂的去除方法[EB/OL].[2005-09-19].http://www.poultryinfo.org/nutritionchannel/show Artide.asp Artide ID=22078.
  • 2RYAN C A.Biochemistry of Plants[M].Edited by Marcus A.New York:Acdemic Press,1981(6):351-370.
  • 3KIM S H,HARA S,HASE S,et al.Comparative study on amino acid sequence of Kunitz-type soybean trypsin inhibitor,Tia,Tib,Tic[J].Journal of Biochemistry,1985,98:435-448.
  • 4LASKOWSKI M,J R,KATO I.Protein inhibitors of proteinase[J].Annual Review of Biochemistry,1980,49:593-629.
  • 5HILDER B A,GATEHOUSE A M R,SHEERMAN S E,et al.A novel mechanism of insect resistance engineered into tobacco[J].Nature,1986,330:160-163.
  • 6RYAN C A.Protease inhibitors in plants-genes for improving defenses against insects and pathogens[J].Annual Review of Phytopathology,1990,28:425-449.
  • 7PARK D S,GRAHAM M Y,GRAHAM T L.Identification of soybean elicitation competency factor,CF-1,as the soybean Kunitz trypsin inhibitor[J].Physiological and Molecular Plant Pathology,2001,59:265-273.
  • 8TRUEMPER S,FOLLMAN H,HAEBERLEIN I.A novel dehydroascorbate reductase from spinach chloroplasts homologous to plant trypsin inhibtor[J].FEBS Letters,1994,352:159-162.
  • 9WELLES J M,STRICKLAND S.Aprotinin,a Kunitz-type protease inhibitor,stimulates skeletal muscle differentiation[J].Development,1994,120:3639-3647.
  • 10MORELL S,FOLLMANN H,DETULLIO M,et al.Dehydeoascrobate and dehydroascrbate reductase are phantom indicators of oxidative stress in plants[J].FEBS Letters,1997,414:567-570.

二级参考文献16

  • 1Darviall A. G. , Albersheim, P. Phytoalexins and their elicitors-A defense against microbial infection in plants [J]. Ann. Rev.Plant Physiol. 1984,35: 243- 275.
  • 2Ayers A. R. , Ebel J. ,Finelll F. ,et al. Host-pathogen interactions IX quantitative assays of elicitor activity and characterization of the elicitor present in the extracellular medium of cultures of Phytophthora megasperma vat. sojae [J]. Plant Physiology 1976,57:751-759.
  • 3Bhattacharyya M.K. Ward E. W. B. Temperature-induced susceptibility of soybean to Phytophthora megasperma f. sp. glycines: phenylalalanine ammonia- lyase and glyceollin in the host:growth and glyceollin I sensitivity of the pathogen[J]. Physiological and Molecular Plant Pathology, 1987, 31 : 407-419.
  • 4Moesta P. ,Grisebach H. Effects of biotic and abiotic elicitors on phytoalexon metabolism in soybean[J]. Nature, 1980,286: 710 -711.
  • 5Hahn, M.G., Bonhoff, A., Grisebach, H. Quantitative localization of the phytoalexin glyceollin I in relation to fungal hyphae in soybean roots infected with Phytophthora megasperma f. sp.glycinea [J]. Plant Physiology, 1985,77: 591- 601.
  • 6Ingham, J. L., Keen, N.T., Mulheirn, L.J. ,et al. Inducibly-formed isoflavanoids from leaves of soybean [J]. Phytochemistry,1981,20: 795-798.
  • 7Ward E. W. B. , Lazarovits G. , Unwin C. H. , et al. Hypocotyl reactions and glyceollin in soybean inoculated with zoospores of phytophthora megasperma vat. sojae [J]. Phytopathology, 1979,69(9) :951-954.
  • 8Welle, R. , Grisebach, H. Induction of phytoalexin synthesis in soybean:enzymatic cyclizatlon of prenylated pterocarpans to glyceollin isomers [J]. Archives of Biochemistry and Biophysics,1988,263(1):191-198.
  • 9Lyne R. L. , Mulheirn L.J. Mino pterocarpinioids of soybean[J].Tetrahedron letter. 1978:3127-3128.
  • 10Weinstein 1. I. , Hahn M.G. , Albersheim P. Host- pathogen interactions XVIII. Isolation and biological activity of glycinol, a pterocarpan phytoalexin synthesized by soybeans[J]. Plant Physiology, 1981,68 : 358- 363.

共引文献6

河北农业大学学报
2007年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部