半导体激光诱发脉络膜新生血管的研究

Experimental Study of Choroidal Neovascularization Induced by Semiconductor Laser

目的研究脉络膜新生血管的发生机制。方法用半导体激光建立脉络膜新生血管的动物模型,造模后1、2、3、4、8周行荧光素眼底血管造影检查,8周行光学相干断层扫描检查,HE染色观察。结果半导体激光造模后2周,荧光素眼底血管造影时开始渗漏,4周时渗漏达到高峰,8周时趋于稳定。造模后8周,光学相干断层扫描检查、HE染色可见脉络膜新生血管。结论半导体激光可诱导脉络膜新生血管动物模型,该模型可作为人类脉络膜新生血管模型进行实验研究。

Objective To study the pathogenesis of choroidal neovascularization （CNV）. Methods CNV an.imal models were induced with semiconductor laser, then the fundus fluorescein angiography （FFA） examination occurred after 1, 2, 3, 4 and 8 week respectively, the optical coherence tomography （OCT） examination occurred just before euthanasia and tissue was processed for hematoxylin eosin stain in 8th week. Results The leakage of CNV was firstly observed in 2nd week by FFA, the highest rate was observed on week 4 till to week 8. CNV was confirmed by OCT and hematoxylin eosin stain. Conclusion The model induced by semiconductor laser can be us ed as an animal model for experimental study of CNV.