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固定化肌红蛋白的类酶活性电化学研究 被引量：2

Electrochenmical research on enzyme-like activities of immobilzed myoglobin
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摘要 Myoglobin(Mb)was immobilized’on glassy carbon electrode(GCE)surface by carl·ageenan.The enzyme-Iike properties of immobilized Mb,such as catalytic reduction of peroxides(hydrogen peroxide,cumene hydroperoxide,t-butyl hydroperoxide,2-butanone peroxide),and dehalogenation of haloethanes(hexachloroethane,pentachloroethane,tetrachloroethane),were explored by cyclic voltammetry.Stability and reproducibility of the modified electrode make it possible to determine the substances. Myoglobin （Mb）was immobihzedbn glassy carbon electrode （GCE）surface by carl · ageenan. The enzyme -Iike properties of immobilized Mb, such as catalytic reduction of peroxides （hydrogen peroxide, cumene hydroperoxide, t -butyl hydroperoxide, 2 - butanone peroxide）, and dehalogenation of haloethanes （ hexachloroethane, pentachloroethane, tetrachloroethane）, were explored by cyclic voltammetry. Stability and reproducibility of the modified electrode make it possible to determine the substances.
作者 彭月敏 王启会 刘慧宏
机构地区 襄樊学院化学与生物科学系湖北
出处 《化学研究与应用》 CAS CSCD 北大核心 2008年第6期740-744,共5页 Chemical Research and Application
基金 湖北省自然科学基金项目(2005ABA007) 湖北省教育厅科技基金项目(G200525001)
关键词 肌红蛋白 电化学 类酶活性 过氧化物 氯化乙烷 myoglobin electrochemistry enzyme-like activity peroxides chloroethanes
分类号 Q51 [生物学—生物化学] S816.79 [农业科学—饲料科学]
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参考文献16

  • 1Ozaki S, Matsui T, Roach M P, et al. Rational molecular design of a catalytic site:engineering of catalytic function to myoglobin active site framework [ J ]. Coord Chem Rev, 2000,198:39 - 59.
  • 2刘慧宏,庞代文.氧化还原蛋白质电化学研究[J].化学进展,2002,14(6):425-432. 被引量：19
  • 3Hu Y, Hu N, Zeng Y. Electrochemistry and electrocatalysis with myoglobin in biomembrane - like surfactant - polymer 2C12N + PA - composite films [J]. Talanta ,2000 ,50 : 1183 - 1195.
  • 4Lvov Y M, Lu Z, Schenkman J B, et al. Direct electrochemistry of myoglobin and cytochrome P450cam in ahemat later - by - layer films with DNA and other pollyions [ J ] . J Am Chem Soc, 1998,120:4073 - 4080.
  • 5Wang Q, Lu G, Yang B. Myoglobin/sol - gel film modified electrode : direct electrochemistry and electrochemical catalysis [ J ]. Langmuir, 2004,20 ; 1342 - 1347.
  • 6Li Q, Luo G. Feng J. Direct electron transfer for heme proteins assembled on nanocrystalline TilO2 film [ J ]. Electroanalysis ,2001,13:359 - 363.
  • 7李业梅,刘慧宏,张志凌.肌红蛋白在海藻酸钠水凝胶中的电化学和电催化特性[J].化学研究与应用,2004,16(4):530-532. 被引量：2
  • 8Csoregi E ,Gorton L, Marko- Varga g, et al. Peroxidase -modified carbon fiber microelectrodes in flow- through detection of hydrogen peroxde and organic peroxides [ J ]. Anal Chem, 1994,66:3604 - 3610.
  • 9Liu H, Tian Z, Lu Z, et al. Direct electrochemistry and electrocatalysis of heme - proteins entrapped in agarose hydrogel films biosens [ J ]. Bioelectron, 2004, 20:294 -304.
  • 10Tsai W C, Cass A E G. Ferroeene - modified horseradish peroxidase enzyme electrodes. A kinetic study on reactions with hydrogen peroxide and lionleie hydroperoxide [ J ]. Analyst, 1995,120 : 2249 - 2254.

二级参考文献11

共引文献19

同被引文献27

  • 1刘慧宏,万永清,陈显堂,邹国林.固定化辣根过氧化物酶在有机溶剂/水混合溶液中的电化学[J].物理化学学报,2006,22(7):868-872. 被引量：6
  • 2Zaks A, Klibanov A M. Enzymatic catalysis in organic media at 100 degrees C[J]. Science,1984,224:1249-1251.
  • 3Fitzpatrick P A, Steinmetz A C U, Ringe D, et al. Enzyme crystal structure in a neat organic solvent[J]. Proceedings of the National Academy of Sciences of the USA,1993,90: 8653-8657.
  • 4Schmitke J L, Wescott C R, Klibanov A M. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents[J]. Journal of the American Chemical Society,1996,118:3360-3365.
  • 5Burke P A, Griffin R G, Klibanov A M. Solid-state NMR assessment of enzyme active center structure under nonaqueous condition[J]. Journal of Biological Chemistry, 1992,267:20057-20064.
  • 6Affleck R, Haynes C A, Clark D S. Solvent dielectric effects on protein dynamics[J]. Proceedings of the Nation- al Academy of Sciences,1992,89:5167-5170.
  • 7Schmitke J L, Wescott C R, Klibanov A M. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents[J]. Journal of the American Chemical Society,1996,118:3360-3365.
  • 8Wang Y, Guo Y Z, Zhu G Y, et al. Cryoimmobilized enzyme for biosensor construction[J]. Sensors and Actuators B,1996,31:193-196.
  • 9Burke P A, Griffin R G, Klibanov A M. Solid-State nuclear magnetic resonance investigation of solvent dependence of tyrosyl ring motion in an enzyme[J]. Biotechnology and Bioengineering,1993,42:87-94.
  • 10Cao S G, Feng Y, Liu Z B, et al. Lipase catalysis in organic solvents[J]. Applied Biochemistry and Biotechnology, 1992,32:7-13.

引证文献2

化学研究与应用
2008年 第6期

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