三羟异黄酮对BALB／c-3T3细胞间隙连接通讯的影响

Effects of Genistein on Gap Junctional Intercellular Communication of BALB/c-3T3 Cells'

[目的]研究三羟异黄酮(GEN)对细胞间隙连接通讯的影响。[方法]采用荧光漂白后恢复(fluorescence redistribution after photobleaching,FRAP)技术,在激光扫描共聚焦显微镜(LSCM)下观察GEN于浓度为0、12.5、50、100μmol/L时对BALB/c-3T3细胞荧光漂白后恢复的影响。[结果]受试细胞在GEN 12.5μmol/L时经漂白后荧光恢复率为(36.13±5.43)%,与对照组差异无统计学意义;但在50、100μmol/L时经漂白后荧光恢复率分别为(32.93±5.06)%和(28.18±10.69)%,比对照组明显降低。[结论]GEN在较高剂量时抑制细胞间隙连接通讯(gap junctional intercellular communication,GJIC)功能,提示它在一定条件下可能有促癌作用。

[ Objective ] To study the effects of Genistein（ GEN ）on gap junctional intercellular communication（ GJIC ）. [ Methods ] BALB/c-3T3 cells were exposed to GEN at the concentrations of 0, 12.5, 50, 100μmol/L, the effects of GEN on GJIC was observed under the laser scanning confocal microscope（ LSCM ） using fluorescence redistribution after photobleaching （ FRAP ）. [ Results ] The fluorescence recovery of cells treated with 12.5 μmol/L GEN was （ 36.13 ± 5.43 ） %, no significant difference compared with control; but the fluorescence recoveries of cells treated with 50 or 100 μmol/L GEN were （ 32.93 ± 5.06 ）% and （ 28.18 ± 10.69 ）% respectively, significantly reduced, compared with control. [ Conclusion ] GEN significantly inhibits GJIC function at higher concentrations, indicating it could promote cancer under certain conditions.