二倍体大白菜与四倍体结球甘蓝杂种的获得及其SSR鉴定与GISH分析

Production and SSR Identification and GISH Analysis of Interspecific Hybrids between Diploid Chinese Cabbage and Tetraploid Cabbage

为创制结球甘蓝-大白菜异附加系、异代换系、易位系,利用二倍体大白菜(AA,2n=20)为母本与四倍体结球甘蓝(CCCC,2n=36)杂交,通过胚挽救获得了异源三倍体杂交种(ACC,2n=28);利用SSR对杂交种进行鉴定,6株F1均能扩增出父本的特征带,表明这6株是真杂交种。异源三倍体基因组原位杂交结果表明,未加封阻时,大白菜与结球甘蓝染色体均有较强的杂交信号;当加入适当比例的封阻时,来自大白菜和结球甘蓝染色体上的杂交信号强弱有明显差别;封阻过度时,大白菜与结球甘蓝染色体的杂交信号都十分微弱。说明大白菜与结球甘蓝基因组具有较高的同源性。

Allotriploid hybrids（ACC, 2n=3x=28）were produced by crossing diploid Chinese cabbage as female parent （Brassica campestris L. ssp. pekinensis, AA, 2n=2x=20） with tetraploid cabbage （B. oleracea var. capitata L., CCCC, 4n=4x=36） by means of embryo rescue, which will be used for constructing cabbage-Chinese cabbage alien addition lines, substitution lines and translocation lines. The hybrids were identified by SSR, the results demonstrated that 6 F1 plants were all interspecific hybrids because they could be amplified the character bands from male parent. The results of GISH in allotriploid hybrids showed when blocking DNA was empty, strong signals were detected in each chromosome of Chinese cabbage and cabbage. When blocking DNA was moderate, the intensity of signals in the chromosomes between Chinese cabbage and cabbage was obviously different. When blocking DNA was excess, weak signals were detected in each chromosome of Chinese cabbage and cabbage. These results indicated that high homologization between Chinese cabbage and cabbage was involved.