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油菜细胞质雄性不育相关基因的筛选 被引量：6

Screening of the new gene related to CMS in Brassica napus
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摘要 甘蓝型油菜波利马(Brassica napuspol.)细胞质雄性不育系是一种很有实用价值的油菜不育类型.目前关于其分子机制的研究已证实,线粒体ATP合成酶的一个亚基ATP6与其雄性不育相关.本实验以ATP6作为诱饵蛋白,应用酵母双杂交共转化的方法筛选与ATP6相互作用的蛋白质.在获得的阳性克隆中,发现了一个与tAPX基因及其表达的蛋白都具有较高同源性.它是活性氧清除的重要酶类,很可能与细胞质雄性不育间建立起了某种联系. The cytoplasmic male sterile （CMS） line of Brassica napus pol. is of great research utility among the rapeseed sterile lines. Researches of its molecular mechanism has substantiated that ATP6, subunit of the ATP synthase, is related with CMS. By using of yeast two hybrid system, ATP6 was chosen as bait protein to screen its interacting partners. A fragment has high identity with the cDNA sequence of tAPX and its coded protein, tAPX is a sort of APX enzymes that scaveng reactive oxygen species （ROS）. The research might suggest its possible role in CMS and flower development.
作者 向俊蓓 刘志斌 王艳 岳渝飞 王健美 李旭锋 杨毅
机构地区 四川大学生命科学学院生物资源与生态环境教育部重点实验室
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2008年第3期640-644,共5页 Journal of Sichuan University(Natural Science Edition)
基金 国家自然科学基金(30570986)
关键词 酵母双杂交 细胞质雄性不育 ATP6 tAPX H2O2 yeast two hybrid （Y2H）, cytoplasmic male sterility （CMS）, ATP6, tAPX,H2O2
分类号 Q75 [生物学—分子生物学]
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参考文献17

  • 1Budar F, Touzet P, de Paepe R. The nucleo-mitochondrial conflict in cytoplasmic male sterilities revisited[J ]. Genetica, 2003, 117: 3.
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二级参考文献4

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共引文献22

同被引文献82

引证文献6

二级引证文献35

四川大学学报（自然科学版）
2008年 第3期

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