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截短侧耳素产生菌的鉴定及发酵培养基初筛 被引量：4

Identification of a pleuromutilin-producing fungus and primary screening of fermentation medium
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摘要 对产截短侧耳素(pleuromutilin)的真菌P4-04进行形态学观察和ITS保守序列鉴定,初步鉴定P4-04可能属于担子菌纲斜盖菇属新种.采用单因子实验对发酵培养基条件筛选.优化结果显示,最佳碳源为果糖,浓度为5%,最佳氮源为2%的黄豆饼粉,FeSO4添加浓度为0.04%.在此条件下获得的产量比原始条件提高73.1%. A fungil P4-04 producing pleuromutilin is identified by morphological observation and ITS conserved sequence BLAST. Primary identification shows that P4-04 may belong to Basidiomycetes, Clitopilus sp. Single-factor test is used to screening the fermentation medium. The optimal results suggest that the best carbon source is 5 % flucose; the best nitrogen source is 2 % Soybean cake powder, and the concentration of FeSO4 is 0.040%. Production in final medium increases 73.1% compared with the primary.
作者 黄宇琪 张怡 胡昌华
机构地区 西南大学药学院现代生物医药研究所
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2008年第3期699-702,共4页 Journal of Sichuan University(Natural Science Edition)
基金 重庆市科技攻关项目(2003-8110)
关键词 截短侧耳素产生菌 鉴定 单因子实验 发酵 Pleuromutilin-producer, identification, single-factor test, fermentation
分类号 Q93-331 [生物学—微生物学]
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参考文献12

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二级参考文献33

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共引文献27

同被引文献36

  • 1赵凯,周东坡,平文祥,刘军,马玺,金涛.产紫杉醇菌株原生质体诱变育种的研究[J].生物工程学报,2005,21(5):848-851. 被引量：23
  • 2邹祥.高效液相色谱法测定发酵液中截短侧耳素的含量[J].食品与发酵工业,2006,32(3):83-85. 被引量：5
  • 3Kavanagh F, Hervey A, Robbins W J. Antibiotic substances from Basidiomycetes: VIII. Pleurotus multilus (Fr.) Sacc. and Pleurotus passeckerianus Pilat [ J]. Proc Natl Acad Sci U S A, 1951, 37(9) : 570 -574.
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引证文献4

二级引证文献11

四川大学学报（自然科学版）
2008年 第3期

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