摘要
目的建立犬血浆中格列齐特浓度的HPLC测定法。方法采用色谱柱为Nucleodur C18硅胶柱（250×4.6mm,5um）;流动相为磷酸盐缓冲液（pH2.5）-甲醇（40∶60,v/v）;流速1.0mL/min;检测器波长228nm;以甲苯磺丁脲为内标。结果格列齐特浓度在0.125-16μg/mL范围内线性关系良好（r=0.9999）,最低检测浓度为0.10ug/mL,日间及日内RSD均小于8%,平均萃取回收率合为79.98%。结论本法操作简便、快速、灵敏度高,可用于格列齐特药代动力学研究。
Objective To establish high performance liquid chromatography （HPLC） method for the determination of the content of gliclazide in dog plasma. Methods The chromatographic conditions were as follow： Nucleodur C18 kieselgel column （250×4. 6 mm2, φ5μm） as chromatographic column; phosphate buffer （pH2.5） methanol （40：60,v/v） as mobile phase; the flow rate： 1.0 mL/min;the detection wavelength： 228 nm; tolbutamide selected as an internal standard. Results The calibration curve was linear （r=0. 999 9） in the concentration range of 0. 125-16μg/mL for gliclazide. The limit of detection （LOD） was 0.10 μg/mL. The RSD of inter- and intra-day were less than 8%. The average extraction recovery rate was 79.98%. Conclusion HPLC assay is simple, rapid and sensitive, suitable for the pharmacokinetic study of gliclazide.
关键词
格列齐特
色谱法
高效液相
血药浓度
犬
gliclazide
stratography
high performance liquid chromatography （HPLC）
plasma concentration
dog
