TrkB和BDNF在胃癌组织中的表达及意义

Expression and significance of tyrosine kinase receptor B and brain-derived neurotrophic factor in gastric carcinoma

目的观察酪氨酸激酶受体B（TrkB）及其配体脑源性神经营养因子（BDNF）在胃癌组织、癌旁组织和正常胃黏膜组织中的表达情况，探讨TrkB和BDNF与胃癌临床病理参数的关系。方法采用免疫组织化学SP法检测64例原发性胃癌组织、癌旁组织，34例有淋巴结转移患者中对应的阳性淋巴结和20例正常胃黏膜组织中TrkB和BDNF的表达，分析其与临床病理特征的关系。结果胃癌组织中TrkB和BDNF阳性表达率分别为60．9％、59．4％，而癌旁组织和正常胃黏膜组织中无一例表达。TrkB和BDNF表达与患者性别、年龄、肿瘤分化程度等无关，而与浸润深度、淋巴结转移和TNM分期有关。浸润至胃壁全层、有淋巴结转移和TNM分期Ⅲ～Ⅳ期的TrkB和BDNF阳性表达率明显高于未浸润至胃壁全层、无淋巴结转移和TNM分期Ⅰ～Ⅱ期（P〈0．01）。胃癌转移患者TrkB和BDNF在淋巴结转移灶中的阳性表达率（76．5％，26／34；70．6％，24／34）较原发灶（85.3％，29／34 82.4％，28／34）低，但两者比较差异无统计学意义（P〉0．05）。结论TrkB和BDNF表达与胃癌发生、发展密切相关，二者的表达增强提示肿瘤可能已发生局部侵袭和远处转移。

Objective To investigate the expression＇s of tyrosine kinase receptor （Trk）B and brain--derived neurotrophic factor （BDNF） protein in human gastric carcinoma and compare them with those in epithelial ceUs of normal mucous, in order to evaluate their clinicopathological significance. Method The expressions of TrkB and BDNF protein in tumor tissues, matched with para-tumor mucosal tissues from 64 cases with gastric carcinoma and normal mucous of 20 cases were observed immunohistochemically and related to some of the clinicopathological parameters. Results The positive rates of TrkB and BDNF protein in tumor tissues were 60.9% and 59.4% respectively, but there was negative in matched para-tumor mucosal tissues and normal mueosal tissues. TrkB and BDNF protein expressions were related to invasive depth, lymph node metastasis and TNM stage of cancer, but not to sex, age and degrees of cancerous differentiation. The positive rates of TrkB and BDNF protein in cases with serosal infiltration, lymph node metastasis and TNM stage Ⅲ-Ⅳ were significantly higher than those in cases without serosal infiltration, lymph node metastasis, and TNM stage Ⅰ - Ⅱ （P〈 0.01 ）. In group with metastasis the positive rates of TrkB and BDNF protein were lower in metastatic foci （76.5%, 26/34, 70.6%, 24/34） than those in primary tumor （85.3%, 29/34, 82.4%, 28/34）, but statistically there was no significant difference between them （P 〉 0.05）. Conclusions The expressions of TrkB and BDNF protein are closely related to tumorigenesis and progression of gastric carcinoma. The increased expression of TrkB and BDNF may promote the occurrance of local invasion and metastasis of gastric carcinoma