灯盏花注射液对大鼠肺纤维化作用的初步研究

Effect of Breviscapus Injection on Pulmonary Fibrosis in Rats

目的探讨灯盏花注射液对博来霉素诱导的肺纤维化大鼠肺组织氧自由基和显微结构的影响。方法SD大鼠经气管内灌注博莱霉素诱导肺纤维化,随后每日腹腔注射灯盏花注射液4 mL.kg-1,另一组每日注射地塞米松。于注药28 d后处死。通过HE染色和透射电镜检查肺组织,并测定肺组织SOD,MDA来评价干预效果。结果灯盏花组结果与地塞米松组相似,与博莱霉素组相比,肺组织SOD下降,MDA升高,光镜结果提示肺纤维化程度减轻。结论灯盏花注射液能减轻大鼠肺纤维化,部分机制与抗氧化有关。

OBJECTIVE To study the effect of Breviscapus Injection on changes of in oxyradical contents and microstructure of the pulmonary tissue of rats with pulmonary fibrosis induced by pouring bleomycin into the lungs. METHODS Thirty-two SpragueDawley （SD） rats were randomLy divided into four groups ： control, bleomycin （ BLM ） , breviscapus （BRE） and dexamethasone （DXM） group,with 8 rats in each group. After 28 days,the MDA and SOD of lung were measured,and the histology was examined. RESULTS In the BRE group,the concentration of was significantly lower than which of BLM group （P 〈0.01 ） ,and the degree of fibrosis was more mitigative than BLM group when examined by microscope, similar to that in the DXM group. CONCLUSION Breviscapus can reduce the damage caused by oxygen-derived free radicals in bleomycin. Treatment with breviscapus may be useful in inhibiting pulmonary fibrosis.