蒙药童格勒格-1四种粗提物对大鼠肝细胞BRL株低密度脂蛋白受体基因表达的影响

Effects of Four extracts from Mongolia drug TGLG-1 on expression of low density lipoprotein receptor gene in rat hepatocyte strain BRL

目的观察蒙药童格勒格-1(TGLG-1)四种粗提物对大鼠正常肝细胞BRL株低密度脂蛋白受体(LDL-R)基因表达的影响。方法制备出蒙药童格勒格-1的乙醇、正丁醇、乙酸乙酯、石油醚的提取物。体外培养大鼠正常肝细胞BRL株,通过MTT比色法观察蒙药童格勒格-1提取物对大鼠肝细胞体外毒性的量效关系,确定合适的用药浓度。将大鼠肝细胞分为5组,一组为对照组,其余四组为给药组。将四种粗提物以一定的给药浓度与正常大鼠肝细胞培养24 h后,用RT-PCR法测定大鼠肝细胞低密度脂蛋白受体mRNA表达水平。结果蒙药童格勒格-1提取物中正丁醇和乙酸乙酯提取物作用后的大鼠正常肝细胞低密度脂蛋白受体mRNA相对含量高于对照组,具有显著性差异(P<0.01)。乙醇和石油醚提取物与对照组相比,无显著性差异(P>0.05)。结论蒙药童格勒格-1正丁醇和乙酸乙酯提取物能明显增强大鼠正常肝细胞低密度脂蛋白受体基因表达,从而加快血中低密度脂蛋白的清除,起到调控血脂水平的作用。

OBJECTIVE To observe effects of four extracts from Mongolia drug TGLG-1 on the expression of low density lipoprotein receptor（LDL-R） gene in rat hepatocyte strain BRL. METHODS Four extracts form Mongolia drug TGLG-1 by alcohol, n-butyl alcohol,ethyl acetate and petroleum ether were prepared. The rat normal hepatocytes were cultured in vitro. Through observing the relation of the drug concentration and vitro toxicity on the rat hepatocytes by MTT colorimetric method, the appropriate drug concentration to culture the rat hepatocytes was determined. The rat hepatocytes were divided into five groups. One group was cultured without the drug as the control group and the other four groups were cultured with the drug in the appropriate concentration. After the hepatocytes were cultured with four extracts in the appropriate concentration for 24 hours, RT-polymerase chain reaction （RT-PCR） technique was used to observe the mRNA expression of LDL-R in the rat hepatocytes of every group. RESULTS The mRNA expression level in the rat hepatocytes cultured with the drug extracted with n-butyl alcohol and ethyl acetate was significantly higher than the control group（P 〈0.01 ）. But the other two groups cultured with the drug extracted with alcohol and petroleum ether have no remarkable difference with the control group （ P 〉 0.05 ）. CONCLUSION The extracts from Mongolia drug TGLG-1 by n-butyl alcohol and ethyl acetate can increase the LDL-R mRNA expression level in the rat hepatocytes remarkably, so it can promote the metabolism of the low density lipoprotein to adjust the the blood-lipid level.