HPLC测定脉舒胶囊中木犀草素的含量

Quantitative Determination of Luteolin in Maishu Capsules by HPLC

目的建立高效液相色谱法测定脉舒胶囊中木犀草素的含量。方法用ZORBAX SB-C18（4．6mm×250mm，5μm）色谱柱，甲醇-0．4％磷酸溶液（55：45）为流动相，流速1．0mL·min^-1；检测波长：350nm；柱温：35℃。结果木犀草素的进样量在0．003864～0．02318μg范围线性关系良好（r=0．9999），平均加样回收率为98．31％（RSD=1．15％），符合分析要求。结论对样品的提取条件和测定条件进行了优选，为脉舒胶囊中木犀草素的测定提供了一种准确可靠的HPLC测定方法，可更好的控制其质量。

OBJECTIVE To determine the content of luteolin in maishu capsules by HPLC. METHODS The analysis was carried out on a Hyper ZORBAX SB - C 18 column （4.6 × 250 mm,5 μm）. The mobile phase was methanol-0.4% H3 PO4 （ 55：45 ） ; Flow - rate was 1.0m L · min^-1. Wave - length was 350nm. The temperature was at 35℃. RESULTS The method was simple. The liner range of luteolin was 0. 003 864 - 0. 023 18μg and the correlation （r） was 0.999 9. The recovery was 98.31% with RSD 1.15%. CONCLUSION The method is easy to determine the content of maishu capsules.