摘要
目的探讨葡萄糖-6-磷酸异构酶、国产抗CCP抗体对类风湿关节炎(RA)的诊断价值,与德国欧蒙抗环瓜氨酸肽抗体(抗CCP抗体)之间的相关性以及在临床诊断上的意义。方法对165例RA患者血清和161例非RA患者血清和32例正常人血清用三种酶联免疫吸附试验(ELLSA)进行检测。结果165例RA患者葡萄糖-6-磷酸异构酶敏感度和特异度分别为71.5%和97.9%;国产抗CCP对RA的敏感度和特异度分别为72.1%和81.9%;与非RA对照组和正常对照组比较差异有统计学意义(P<0.05);葡萄糖-6-磷酸异构酶与德国欧蒙抗CCP抗体和国产抗CCP抗体重叠阳性率分别为66.7%和77.8%。结论葡萄糖-6-磷酸异构酶与德国欧蒙抗CCP抗体、国产抗CCP抗体均能能用于RA的早期诊断,最好是多个指标综合判断。
Objective To investigate the value of Glucose-6-phosphate Isomeras( GPI)in the diagnosis of rheumatoid arthritis ( RA ) and compare the relativity of Glucose-6-phosphate Isomeras (GPI) with china' s anti- cyclic citrullinated peptide (CCP) antibody, and EUROIMMUN ' s anti-cyclic citrullinated peptide (CCP) anti- body. Methods Serum samples from 165 RA patients and 161 no-RA patients and 32 normal control subjects were tested by enzyme-linked immunosorbent assay ( ELISA ). Results The sensitivity and specificity of Glucose-6-pbospbate lsomeras(GPI) in the 165 RA patients were 71.5% and 97.9% respectively; The sensitivity and specificity of china' s anti-cyclic citrullinated peptide(CCP) antibody were 72. 1% and 81.9% respectively; there were significant differences between test group and control group ( P 〈 0. 05 ) ; The cross positive rate of Glucose-6-phosphate Isomeras ( GPI ) with China ' s anti-CCP, EUROIMMUN ' s anti-CCP were 66. 7% and 77.8% respectively. Conclusion The Glucose-6-phosphate lsomeras(GPI) and China' s anti-CCP assay might be used as a valuable index in the early diagnosis of RA. The many test synthetic judgment should be better.
出处
《中国实用医药》
2008年第18期7-9,共3页
China Practical Medicine
基金
河南省教育厅自然科学研究项目(项目编号:2007320020)
关键词
葡萄糖-6-磷酸异构酶
抗环瓜氨酸肽
抗体
类风湿关节炎
酶联免疫吸附试验
Glucose-6-phosphate isomeras ( GPI )
Anti-cyclic citrullinated peptide
Antibody
Rheu- matoid arthritis
Enzyme-linked immunosorbent assay