3种临床常用冠桥材料对体外培养鼠成纤维细胞毒性的比较

Cytotoxicity of three kinds of temporary crown and bridge materials on mouse fibroblasts in vitro

目的:观察比较临床常用3种暂时冠桥材料的细胞毒性。方法:应用体外细胞培养琼脂覆盖法和台盼蓝染色法观察细胞反应和细胞染色情况,以评价自凝塑料、热凝塑料、DMG-TEMP树脂3种常用暂时冠桥材料的细胞毒性。结果:3种常用暂时冠桥材料周围和材料下褪色区范围及褪色区内细胞反应评价分别是自凝塑料2/3,中度细胞毒性;热凝塑料1/0,极轻微细胞毒性;DMG树脂1/2,轻中度细胞毒性。台盼蓝染色结果显示,自凝塑料组细胞染色最多(P<0.05),热凝塑料组与DMG树脂组细胞染色无显著差别(P>0.05)。结论:自凝塑料的细胞毒性为中度,临床对活髓牙行暂时冠桥修复时应慎用此材料。

PURPOSE： This study was to investigate the cytotoxicity in vitro of three kinds of common temporary crown and bridge materials, i.e.,self-curing resin, heat-curing resin, and DMG-TEMP hi-acrylic composite. METHODS： L-929 mouse fibroblasts were used to evaluate the cytotoxicity of three kinds of temporary crown and bridge materials by agar overlay assay to test the cell death rate through trypan blue dyeing. RESULTS： The agar overlay assay showed that the value of Zone/Lysis of serf-curing resin was 2/3, heat-curing resin was 1/0, and DMG-TEMP bi-acrylic composite was 1/2. Self-curing resin caused the most significant cytotoxicity （P〈0.05）, followed by heat-curing resin and DMG-TEMP composite, and the latter two showed no significant difference （P〉0.05）. The results of trypan blue dyeing were in accordance with the above. CONCLUSIONS： Self-curing resin, heat-curing resin, and DMG-TEMP bi-acrylic composite could decrease L-929 fibroblasts proliferation. Of all, self-curing resin caused most marked cytotoxicity, so we should avoid using self-curing resin as temporary crown and bridge to protect pulp.