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眼镜蛇毒中降压因子的提取及生物活性的研究 被引量:2

Purification and Biological Activity of Hypotensive Factor from Naja naja atra Venom
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摘要 目的:从广西眼镜蛇蛇毒中分离纯化血管紧张素转换酶抑制剂(angiotensin converting enzyme inhibitor,ACEI),命名为降压因子(hypotensive factor,HF),并测定其生物活性。方法:采用Sephacryl S-100凝胶过滤,CM Sepharose F.F.离子交换层析分离纯化HF,高效液相鉴定纯度,SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定其分子量,紫外分光光度法测定HF对血管紧张素转换酶(ACE)的抑制活性。用离体兔子十二指肠平滑肌测定HF增强缓激肽(bradykinin,BK)的效应。结果:纯化的广西眼镜蛇蛇毒HF经SDS-PAGE检测显示单一条带,测得其相对分子量约为8.2kDa,由12种氨基酸组成,蛋白回收率为5.70%。HF对ACE有明显的抑制作用,其抑制作用与剂量呈正相关。IC50为1.02μg/ml。HF能增强BK对离体兔子十二指肠平滑肌的收缩效应。结论:成功地从广西眼镜蛇蛇毒中纯化出降血压成分。该成分与血管紧张素转换酶抑制剂作用相似,对血管紧张素转换酶有明显的抑制作用。 Objective: To separate and purify the Angiotensin Converting Enzyme Inhibitor (ACEI) named hypotensive factor(HF) from Naja naja atra venom and determine its biological activity. Methods: HF of venom extract was isolated and purified by Sephacryl S-100 gel filtration, and CM Sepharose F.F. ionexc snake hange chromatography respectively. The purified product was identified by HPLC. The molecular weight was determined by SDS-polyacrylamid gel electrophoresis. The ACE inhibitor activity was determined by UV spectrophotometer. The effect of HF on contraction by BK was tested by using isolated intestinal smooth muscle from rabbit. Results: SDS-PAGE showed a single band of HF and a molecular weight of 8.2kDa. HF was a single polypeptide chain and consisted of 12 amino acids. Recovery rate of protein was 5.70%. ACE was inhibited by HF obviously. The inhibitory rate has a positive correlation with the concentration of HF. The IC50 was 1. 02μg/ml. HF could strengthen the contraction of isolated intestinal smooth muscle from rabbit by BK. Conclusion: The method has been proved to be successful for the purification of HF from Naja naja atra venom. HF has a similar effect as Angiotensin Converting Enzyme Inhibitor, and shows a significant inhibitory effect on ACE.
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2008年第6期37-41,共5页 China Biotechnology
基金 广西科技厅科学基金资助项目(桂科自0447059)
关键词 广西眼镜蛇 蛇毒 分离纯化 血管紧张素转换酶抑制剂 降压因子 Naja naja atra Snake venom Separate and purify Angiotensin converting enzyme inhibitor hypotensive factor
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  • 1Ferreira L A, Alves W E, Lucas M S. Isolation and characterization of a bradykinin potentiating peptide (BPP-S) isolated from Scaptocosa raptoria venom. Toxicon, 1996, 34 (5) :599-603
  • 2Mirian A F, Hayashi Antonio C M. The Bradykinin-potentiating peptides from venom gland and brain of Bothrops jararaca contain highly site specific inhibitors of the somatic angiotensin- converting enzyme. Toxicon ,2005,45 (8) : 1163 - 1170
  • 3Komori Y, Sugihara H. Characterization of a new inhibitor for angiotensin converting enzyme from the venom of Vipera aspis. Int J Biochem,1990,22(7) :767-771
  • 4蒋菁莉,任发政,诸晓强.血管紧张素转化酶抑制剂体外活性检测方法的改进及应用[J].中国乳品工业,2006,34(7):9-12. 被引量:14
  • 5何子安 戚挺华 曾广信 等.中国蝮蛇蛇毒中舒缓激肽增强肽的研究[J].生物化学与生物物理进展,1981,13(5):21-29.
  • 6Hayashi M A, Murbach A F, Ianzer D, et al. The C-type natriuretic peptide precursor of snake brain contains highly specific inhibitors of the angiotensin-converting enzyme. J Neurochem,2003,85 (4) :969-977
  • 7韦世秀,刘成军,万瑞融,李牡艳,吴华.广西眼镜蛇毒中降压因子对自发性高血压模型大鼠血压的影响[J].中国药房,2007,18(31):2415-2417. 被引量:1
  • 8韦世秀,舒雨雁,黎红烨.广西眼镜蛇毒磷酸二酯酶分离纯化及部分性质[J].中国生物化学与分子生物学报,2005,21(2):278-281. 被引量:6
  • 9刘绵林,李晓飙,舒雨雁.五步蛇毒中血管紧张素转换酶抑制剂的研究[J].广西医科大学学报,1995,12(3):333-338. 被引量:6
  • 10Fernandez Neshich G, Camargo A C. Using bradykinin- potentiating peptide structures to develop new antihypertensive drugs. Genet Mol Res,2004,3(4) :554-563

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