纳米SiO_2固定化β-葡萄糖苷酶及其在双相体系中水解大豆异黄酮的工艺研究

Immobilization of β-glucosidase with Nano SiO_2 and Its Application in Soybean Isoflavone Hydrolysis in Two-phase-system

纳米材料因其大比表面积、强吸附能力、高机械强度及易分散等特性而备受催化剂载体领域研究者的关注。采用纳米SiO2为载体,通过化学修饰将其用于共价键合法制备固定化酶。发现纳米载体可负载1/8(w/w)的葡萄糖苷酶,且回收率达70%。所得的固定化生物催化剂可用于乙酸乙酯-水双相体系中水解大豆异黄酮,其稳定性比游离酶有明显改善。但纳米颗粒易吸附于界面上,不利于反应结束后两相液滴的聚并及产物的分离。

Nano materials are popular with its interesting properties. Large specific surface area, high mechanical strength, good absorbability make them good potencial carriers of catalyst. With the help of chemical modification, nano SiO2 particals were used as carrier to immobilize 13-glucosidase with covalent binding. It is found that the load of nano particals can reach 1/8 of its weight, and the recovered activity of enzyme is 70%. The resistence of immobilized enzyme to organic solvent wase improved obviously, which was then used in Ethyl Acetate-Water-two-phase-system to hydrolyzed soybean isoflavones.