重组乙型肝炎病毒e抗原的研究进展

Research Progress on Rcombinant Hepatitis B e Antigen

乙型肝炎e抗原(HBeAg)是由HBV DNA C基因区编码的一种非颗粒性的分泌型核壳蛋白,临床上将其作为判断HBV活动性复制的指标之一。HBeAg是重要的生物原材料,广泛用于HBV感染者血清学检测的相关诊断用品的制备。重组HBeAg的表达和纯化技术较为成熟,已经在各种表达系统中成功地表达了目的蛋白。而影响其表达的关键因素包括前C区重要位点变异、载体选取以及RNA干扰(RNAi)的影响作用等。因此,提高重组HBeAg的表达量和纯度,避免与HBcAg交叉反应才能满足相关诊断制品的要求。高质量重组乙肝e抗原的获取,能为改善相关诊断制品奠定物质基础,为开发新型HBV治疗和预防性疫苗提供了依据,同时也为HBeAb检测方法学的优化提供可能。

HBeAg is an ungranular secretory protein, which encoded by C gene of HBV DNA and it increases with the replication of HBV. So it is one of the markers of active replication of HBV in clinical diagnosis. HBeAg is important biologic raw materials which is widely used in preparation of related diagnostic articles with HBV infection serological detection. The technology of expression and purification of recombinant HBeAg is quite mature, which had successfully expressed the target protein in various expression systems. The key factors on HBeAg expression include important site mutation in precore region , the choice of vectors, effects of RNA interference（RNAi） and so on. Therefore, in order to meet requirements of related diagnostic products, it need to improve expression level and purity of recombinant HBeAg and avoid cross-reaction with HBcAg. In a word, it showed that acquisition of high quality recombinant HBeAg could lay substantial foundation for improving diagnostic products, provide a reliable evidence for exploiting new type of therapeutic and preventive HBV vaccine and offer Key possibility of HBeAb detection methodological optimization. words HBeAg Expression Mutation Diagnosis