摘要
本文以培养的中华大蟾蜍（Ｂｕｆｏｂｕｆｏｇａｒｇａｒｉｚａｎｓ）、花背蟾蜍（Ｂｕｆｏｒａｄｄｅｉ）的外周血淋巴细胞为实验材料，采用ＢｒｄＵ标记法和胸苷标记的方法，获得了中华大蟾蜍与花背蟾蜍清晰的高分辨晚复制带，确定了中华大蟾蜍每一染色体的特征性晚复制区；并就中华大蟾蜍与花背蟾蜍的晚复制带纹进行了精确比较，发现中华大蟾蜍与花背蟾蜍的染色体之间具极高同源性。该结果说明：在相同的培养条件下，只要ＢｒｄＵ掺入Ｓ期的时间较为统一，显示复制带的条件基本一致的情况下。
High-resolution late replication banding patterns were obtained on prometaphase chromosomes of Bufo bufo gargarizans and Bufo raddeiby treating cultured lymphocytes with 5-bromodeoxyuridine (BrdU) The characteristic late replication regions on each banded chromosome of Bufo bufo gargarizans, which can be easily distinguished from other chromosomes, have been observed Precise comparison between two species′ highresolution late replication banding patterns indicates that all chromosomes of two species are highly homologous These results show that under the same culture condition and at the same time when BrdU were incorporated into DNA, it is possible to research chromosome homology among different species by comparing replication banding patterns
基金
国家自然科学基金
关键词
中华大蟾蜍
花背蟾蜍
染色体
同源性
Bufo bufo gargarizans,Bufo raddei,Late replication banding pattern,Characteristic late replication region,Chromosome homology
