人重组酸性成纤维细胞生长因子在大肠杆菌中的表达及鉴定

Expression of Human Acidic Fibroblast Growth Factor in E. coli

利用逆转录-DNA聚合酶链式反应，从体外传代培养的人肺成纤维细胞中钓出人酸性成纤维细胞生长因子（aFGF）全编码区的cDNA，将其克隆人pKK223-质粒，在大肠杆菌JM105中高效表达出人重组aFGF。此aFGF经Heparin－Sepharose纯化后，表现了很好的生物学活性。

Using RT-PCR,human acidic fibroblast growth factor(aFGF) cDNA was isolated from human lung fibroblast cultured in vitro,then cloned into pKK223-3 plasmid and expressed in E. coli (JM105 ). After being purified by Heparin-Sepharose chromatography,the recombinant aFGF showed good biological activity.