免疫抑制酸性蛋白的分离纯化及鉴定

Separation,Purification and Characterization of Immunosuppressant Acid Protein

采用卵巢癌腹水为原料，经盐析、DEAE－52、SephadexG100、等电聚焦电泳，Sepharose4B反相免疫亲和层析技术，分离纯化出免疫抑制酸性蛋白（IAP）纯品，经8．0％聚丙烯酰胶凝胶电泳、免疫电泳和双向免疫交叉试验，证实其为单一成分；用此抗原免疫家兔，获得了IAP抗血清，其效价为1：32。

篒mmunosuppressant acid protein was separated and purified from the ascites of patients with oophoroma by salting out method, DEAE-52 and Sephadex G100 column chromatographys,isoe1ectrofocusing electrophoresis and Sepharose 4B reverse immune affinity chromatography. The results of 8.0% polyacrylamide gel, electrophoresis, immunoelectrophoresis and double crossed immunoelectrophoresis proved that the purified IAP was a single component. Anti-IAP serum with a titer of 1:32 was obtained by immunizing rabbits with IAP.