低pH、铝和钙离子对菌根菌赭丝膜伞的毒性和超氧化物歧化酶的影响

Effect of low pH, Al and Ca on toxicity and superoxide dismutase(SOD) activity of Cortinarius russus.

应用急性毒性生物测试方法 ,研究了低 pH和铝离子对菌根菌赭丝膜伞 (Corti nariusrussus)的毒性效应 .试验表明 ,在pH为 6.8、5.5和 4.3,铝离子在低 pH( 4 .3)时对菌根菌毒性最强 ,96hEC50 为 5.0 5mg·L- 1 ,菌根生长受到明显的抑制 ;低 pH和铝离子能诱导菌根菌体内SOD ,与对照组相比 ,菌根菌体内SOD活性明显升高 ;加入钙离子后 ,SOD活性下降 ,并随Ca的投加浓度增加 ,SOD活性逐渐降低 ,表明在低 pH条件下 ,钙离子和铝离子对菌根的影响具有拮抗作用 .

In this paper, acute bioassay method was used to study the toxic effect of low pH and aluminum on mycorrhizal fungi (Cortinarius russus). At pH 4.3, the aluminum toxicity was the strongest, with a 96 h EC 50 of 5.05 mg·L -1 , and the growth of Cortinarius russus was obviously inhibited. The SOD activity of Cortinarius russus was induced by low pH and aluminum, and increased obviously, but decreased when calcium was added, and decreased with increasing calcium concentration. At low pH, there exists an antagonism between calcium and aluminum.