产朊假丝酵母细胞壁33ku蛋白的功能研究

Study on the Function of 33 ku Cell Wall Protein of Candida utilis.

通过胰蛋白酶和枯草杆菌蛋白酶对产朊假丝酵母Candidautilis细胞壁的酶解 ,发现一种分子质量为 33ku的酵母细胞壁主要结构蛋白 .研究显示 ,在细胞壁上这种蛋白质与细胞壁绝大多数蛋白质成分不同 ,它不被胰蛋白酶水解 ,但对枯草杆菌蛋白酶的作用敏感 . 33ku蛋白存在于酵母菌整个对数生长期的细胞壁中 ,特别是在对数早期细胞壁中 ,它是唯一的对胰蛋白酶作用不敏感的蛋白质成分 .实验证明 ,该蛋白质对维系酵母细胞壁骨架成分葡聚糖的相互连接和细胞壁的完整结构 ,具有重要作用 。

By means of proteolysis with trypsin and subtilisin,in the cell wall of Candida utilis , a major structural cell wall protein with molecular mass of 33 ku was found.The 33 ku protein was quite different from most of the other cell wall proteins in Candida utilis. In cell wall it was not hydrolysed by trypsin, but was sensitive to subtilisin. The 33 ku protein was found to exist in cell wall of all logarithmic growth phases. Especially in early logarithmic growth phase, it was proved to be the only protein that was not sensitive to trypsin. The result demonstrated that this protein was important for connection of cell wall skeleton components glucan , and for the intact structure of cell wall. It was an important integral cell wall protein.