氟中毒大鼠肝肾自由基代谢及硒对其影响

Metabolism of Free Radicals and Effects of Selenium in Liver and Kidney of Rats with Chronic Fluorosis.

为探讨硒对氟中毒大鼠肝、肾自由基代谢的影响 ,两组Wistar大鼠饮 1 58mmol/L和2 63mmol/L高氟水 ;饮高氟水的同时加饲 2 0mg/kg硒饲料 ;饮氟水 7个月后加饲硒饲料 .实验 1 4个月时用低温电子自旋共振 (ESR)技术测其肝、肾活性氧自由基 (FR)含量 ;同时测氟 (F)、硒(Se)含量 ;谷胱甘肽过氧化物酶 (GSH Px)、超氧化物歧化酶 (SOD)活性和脂质过氧化物 (LPO)含量 .结果 :氟中毒大鼠在肝、肾氟升高的同时 ,FR和LPO上升 ,GSH Px、SOD下降 .在氟中毒不同时期投硒 ,大鼠肝、肾氟降低 ,FR和LPO减少 ,抗氧化酶活性恢复 .表明硒不但可拮抗大鼠体内的高氟 ,还可纠正高氟造成的自由基代谢紊乱 .

In order to explore effects of senium on metabolism of active oxygen radicals in liver and kidney of rats with fluorosis,2 groups of Wistar rats were fed with normal fodder and high concentration of NaF water (1 58, 2 63 mmol/L fluorine,F).2 groups were fed with selenite fodder (2 0 mg/kg selenium,Se) and high F. At the same time,2 groups were fed with normal fodder and high F for 7 months,then 2 0 mg/kg Se were supplemented in fodder.The contents of free radicals (FR) in liver and kidney were demonstrated by means of the technique of electronic spin resonance (ESR),the contents of Se and F,the activities of glutathion peroxidase (GSH Px), superoxide dismutase (SOD) and the contents of lipid peroxidase (LPO)in liver and kidney were tested in 14 months.The results showed that there was an increase of F,FR and LPO and a decrease of GSH Px and SOD in rats with fluorosis. When Se was given in the different time of fluorosis, F lowered, the signal of FR weekened,LPO lessened,GSH Px and SOD recovered.It indicated that Se not noly could antagonize high F but could rectify metabolic disorders of FR in rats with fluorosis .