运动员剧烈运动后血中应激免疫抑制蛋白的产生

GENERATION OF SUPPRESSIVE PROTEIN AFTER HEAVY PHYSICAL STRESS IN ATHLETES

我们曾经报道，大鼠或小鼠在束缚应激后血中产生了一种能抑制免疫功能的应激免疫抑制蛋白，（又称Ｎｅｕ－ｒｏｉｍｍｕｎｅｐｒｏｔｅｉｎ，ＮＩＰ，神经免疫蛋白）。本工作证明，运动员在大运动量的训练后血清中也产生一种能抑制淋巴细胞转化的物质，它的生化特性及分子量与前述大鼠和小鼠中的应激免疫抑制蛋白相同。在体外实验中，应激大鼠的血清培养人淋巴结细胞，获得了与大鼠实验相同的结果，即人淋巴结细胞也能产生应激免疫抑制蛋白。同时小鼠束缚应激的血清和大运动量的人类血清可以分别抑制人正常淋巴细胞和正常小鼠由ＣｏｎＡ诱导的淋巴细胞转化，以上结果表明，这种应激免疫抑制蛋白的种属特异性不强。

Our previous work showed that under the condition of restraint stress and the control of central nervous system, a suppressive protein (neuroimmune protein, NIP) was generated in peripheral lymph tissue and released into the blood stream, which acts as an immune suppressor. In the present study the serum from the athletes performed a heavy athletic training for 18 days was also able to suppress the lymphocyte proliferation of normal mice. The molecular weight of the suppressive factor in athlete serum determined by gel filtration HPLC was similar to that in mice and rats. In vitro experiment, the normal human lymphocytes were incubated with the serum from restraint stressed rats for 12h and then the extract of human lymphocyte was made for testing the effect on normal lymphocyte proliferation. The result showed that it suppressed normal mouse lymphocyte proliferation. This result was similar to our previous experiment by using normal mouse lymphocyte incubated with the rat serum treated with restraint stress. It was also found that the serum and the extract of lymph node from restraint stressed mice suppressed human lymphocyte proliferation induced by PHA. The results suggest that suppressive protein induced by stress in mice and rats could also be induced in human beings and there is no distinct species specificity among them.