L-arg和LNNA对大鼠胸主动脉内皮损伤后舒缩反应及cGMP含量的影响

EFFECT OF L ARG AND LNNA ON CONTRACTILE RELAXANT RESPONSE AND cGMP LEVEL OF THORACIC AORTA WITH ENDOTHELIAL INJURY IN RAT

本研究在大鼠胸主动脉内皮损伤内膜增生模型上观察了Ｌ－ａｒｇ和ＬＮＮＡ对大鼠胸主动脉条的血管反应性及ｃＧＭＰ含量的影响。血管反应性观察及血管局部ｃＧＭＰ测定发现，大鼠胸主动脉内皮损伤后３天对－ａｒｇ及ＬＮＮＡ的舒缩反应明显受损，血管局部基础ｃＧＭＰ明显下降，用Ｌ－ａｒｇ和ＬＮＮＡ干预后的ｃＧＭＰ变化亦明显受损，损伤后１０天，以上现象明显恢复，损伤后２１天进一步恢复，但仍不能恢复正常。结果表明，内皮损伤后的动脉血管ＥＤＲＦ产生明显受损，且长期不能恢复正常。

In this study,we have observed the effect of L arginine(L arg) and ω N nitro L arginine(LNNA) on the contractile relaxation response and the cGMP level in arterial strip using the model of rat thoracic aorta with endothelial injury and neointimal formation.Organ chamber experiment and radioimmunoassay showed that, the 3rd day after endothelial injury of the rat thoracic aorta,their endothelium dependent relaxtion response caused by L arg,as well as endothelium dependent contractile response caused by LNNA were impaired seriously,basic cGMP reduced obviously,and the change of cGMP caused by L Arg and LNNA was impaired markedly.On the 10th day after injury a pronounced recovery was noticed,and 21st day,the situation was further improved,but could not restore completely.The results indicate that the production of EDRF in the rat thoracic aorta after endothelial injury may be impaired seriously,and could not recover completely for a longer time.